Translation of messenger RNA for histones from HeLa cells by a cell-free extract from mouse ascites tumor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 8 ·1972-08-00 ·Pages 2095-9

Jacobs-Lorena M, Baglioni C, Borun TW

Abstract

7-9S RNA has been isolated from polyribosomes of HeLa S-3 cells in S phase. These RNA species direct the synthesis in vitro of all five classes of human histones when they are added to a protein-synthesizing system derived from mouse ascites tumor cells. The histones synthesized in vitro have been identified by two kinds of acrylamide gel electrophoresis, and by paper electrophoresis of tryptic digests. By use of the ratio of tyrosine to tryptophan incorporation as an index of contamination, it was determined that less than 10% of the 7-9S RNA is translated into proteins other than histones.

MeSH Terms

Amino Acids/metabolism Animals Carbon Isotopes Cell Fractionation Cell-Free System Electrophoresis, Disc Electrophoresis, Paper HeLa Cells/analysis Histones/analysis,biosynthesis Humans Lysine/metabolism Methods Mice Neoplasms, Experimental/metabolism Phenylalanine/metabolism RNA, Messenger/isolation & purification,metabolism RNA, Neoplasm/metabolism Tritium Trypsin Tryptophan/metabolism Tyrosine/metabolism

Chemicals

Amino Acids Carbon Isotopes Histones RNA, Messenger RNA, Neoplasm Tritium Tyrosine Phenylalanine Tryptophan Trypsin Lysine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jacobs-Lorena M

Baglioni C

Borun T W

References (23)

23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.8.2095

Published

1972-08-00

Pages

2095-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426876

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.8.2095 PMC Full Text View journal details

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Translation of messenger RNA for histones from HeLa cells by a cell-free extract from mouse ascites tumor.

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