Home LiteratureArticle Details
PMID: 450708 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

31P NMR studies of the solution structure and dynamics of nucleosomes and DNA.

Nucleic acids research ·Vol. 6 ·No. 4 ·1979-04-00 ·Pages 1607-16

Klevan L, Armitage IM, Crothers DM

Abstract

31P NMR studies of 140 base pair DNA fragments in nucleosomes and free in solution show no detectable change in the internucleotide 31P chemical shift or linewidth when DNA is packaged into nucleosomes. Measurements of 31P spin-lattice relaxation times T1 and 31P-[H] nuclear Overhauser enhancements revealed internal motion with a correlation time of about 4 x 10(-10) sec in double helical DNA, both free in solution and bound to nucleosomal core proteins. This result implies greater dynamic mobility in double helical DNA than has previously been supposed.

MeSH Terms
Chromatin/ultrastructure DNA Deoxyribonucleoproteins Histones Magnetic Resonance Spectroscopy Mathematics Micrococcal Nuclease Nucleic Acid Conformation Nucleoproteins
Chemicals
Chromatin Deoxyribonucleoproteins Histones Nucleoproteins DNA Micrococcal Nuclease
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Klevan L
Armitage I M
Crothers D M
References (21)
21 references, click to expand
  1. The molecular structure of polyadenylic acid.
    J Mol Biol. 1961 Feb;3:71-86 PMID: 13741242
  2. The conformation of DNA and protein within chromatin subunits.
    FEBS Lett. 1977 Oct 1;82(1):63-8 PMID: 913577
  3. 31P magnetic resonance of DNA in nucleosome core particles of chromatin.
    Nature. 1978 Mar 9;272(5649):134-8 PMID: 628441
  4. Phosphorus-31 Fourier transform nuclear magnetic resonance study of mononucleotides and dinucleotides. 2. Coupling constants.
    Biochemistry. 1976 Nov 2;15(22):4860-5 PMID: 990248
  5. Phosphorus-31 Fourier transform nuclear magnetic resonance study of mononucleotides and dinucleotides. 1. Chemical shifts.
    Biochemistry. 1976 Nov 2;15(22):4853-9 PMID: 10961
  6. Electric dichroism studies of the size and shape of nucleosomal particles.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:207-14 PMID: 277341
  7. On the physical basis for ambiguity in genetic coding interactions.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):610-4 PMID: 273223
  8. Transient electric dichroism of rod-like DNA molecules.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):195-9 PMID: 272634
  9. Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin.
    Nucleic Acids Res. 1977 Dec;4(12):4077-89 PMID: 600791
  10. Structure of nucleosome core particles of chromatin.
    Nature. 1977 Sep 1;269(5623):29-36 PMID: 895884
  11. Quantitative determination of mononucleotide conformations in solution using lanthanide ion shift and broadenine NMR probes.
    Nature. 1971 Jul 23;232(5308):236-45 PMID: 4937076
  12. Proton and phosphorus magnetic relaxation studies on the dynamic structure of single-stranded polyriboadenylic acid.
    Biopolymers. 1974 Nov;13(11):2273-80 PMID: 4429782
  13. Quantitative determination of conformations of flexible molecules in solution using lanthanide ions as nuclear magnetic resonance probes: application to adenosine-5'-monophosphate.
    J Mol Biol. 1974 Apr 25;84(4):471-09 PMID: 4840868
  14. 31P magnetic resonance of tRNA.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3482-5 PMID: 242005
  15. Conformational properties of adenylyl-3' leads to 5'-adenosine in aqueous solution.
    Biochemistry. 1976 Feb 24;15(4):756-68 PMID: 1247532
  16. Proton and phosphorus NMR studies of d-CpG(pCpG)n duplexes in solution. Helix-coil transition and complex formation with actinomycin-D.
    Biopolymers. 1976 Mar;15(3):533-58 PMID: 1252592
  17. Temperature dependence of the 31P chemical shifts of nucleic acids. A prode of phosphate ester torsional conformations.
    Biochemistry. 1976 AUG 24;15(17):3796-803 PMID: 952888
  18. Nuclear magnetic resonance studies of the helix-coil transition of poly (dA-dT) in aqueous solution.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):674-8 PMID: 1062778
  19. 31-P chemical shifts in phosphate diester monoanions. Bond angle and torsional angle effects.
    Biochem Biophys Res Commun. 1975 Aug 4;65(3):1073-80 PMID: 239711
  20. Kinky helix.
    Nature. 1975 Jun 12;255(5509):530-3 PMID: 1095931
  21. Conformational study of cyclic and acyclic phosphate esters. CNDO/2 calculations of angle strain and torsional strain.
    J Am Chem Soc. 1976 Mar 31;98(7):1668-73 PMID: 1254849
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.4.1607
Published
1979-04-00
Pages
1607-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327794
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.4.1607 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2