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PMID: 4517937 Published · ppublish English Comparative Study Journal Article

Maternal histone messenger RNA: detection by molecular hybridization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 10 ·1973-10-00 ·Pages 2840-4

Skoultchi A, Gross PR

Abstract

A competition-hybridization assay has been developed for the majority of sequences present in embryonically synthesized histone messenger RNA. The assay permits the first direct demonstration of specific "maternal" messengers in unfertilized sea-urchin eggs. The molecular size of histone messenger RNA stored in the egg appears to be the same as that of histone messenger RNA synthesized by the embryo. Maternal histone messenger RNA is found in the soluble phase of egg homogenates, in the form of ribonucleoprotein particles, unassociated with ribosomes.

MeSH Terms
Animals Base Sequence Cell Fractionation DNA/isolation & purification,metabolism Embryo, Nonmammalian/analysis,cytology,metabolism Female Histones Male Molecular Weight Nucleic Acid Denaturation Nucleic Acid Hybridization Nucleic Acid Renaturation Ovum/analysis,cytology,metabolism Polyribosomes/analysis RNA, Messenger/analysis,biosynthesis,metabolism Sea Urchins Spermatozoa/analysis Tritium
Chemicals
Histones RNA, Messenger Tritium DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Skoultchi A
Gross P R
References (35)
35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.10.2840
Published
1973-10-00
Pages
2840-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427121
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.10.2840 PMC Full Text View journal details
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