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PMID: 4582192 Published · ppublish English Journal Article

Cell-free translation of maternal messenger RNA from sea urchin eggs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 9 ·1973-09-00 ·Pages 2614-8

Gross KW, Jacobs-Lorena M, Baglioni C, Gross PR

Abstract

The template activity of RNA extracted from unfertilized sea urchin eggs was demonstrated in a cell-free translation system, and, for the first time, specific proteins were identified among the products. All messenger RNA activity is recovered, under the conditions used, from the postribosomal supernatant. Histones, among many other proteins, were identified specifically as products of this "maternal" messenger RNA.

MeSH Terms
Animals Aspartic Acid/metabolism Carbon Isotopes Carcinoma, Krebs 2 Cell Fractionation Cell Nucleus/metabolism Cell-Free System Centrifugation, Density Gradient Escherichia coli Female Histones/analysis,biosynthesis Leucine/metabolism Lysine/metabolism Microsomes/metabolism Mitochondria/metabolism Ovum/cytology,metabolism Phenylalanine/metabolism Protein Biosynthesis RNA, Messenger/analysis,metabolism RNA, Ribosomal/metabolism Sea Urchins/metabolism Templates, Genetic Tritium
Chemicals
Carbon Isotopes Histones RNA, Messenger RNA, Ribosomal Tritium Aspartic Acid Phenylalanine Leucine Lysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gross K W
Jacobs-Lorena M
Baglioni C
Gross P R
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.9.2614
Published
1973-09-00
Pages
2614-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427067
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.9.2614 PMC Full Text View journal details
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