Home LiteratureArticle Details
PMID: 4519655 Published · ppublish English Journal Article

Molecular nature of mammalian cell DNA in alkaline sucrose gradients.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 12 ·1973-12-00 ·Pages 3660-4

Simpson JR, Nagle WA, Bick MD, Belli JA

Abstract

Mammalian cell DNA that exhibited anomalous sedimentation in alkaline sucrose gradients was examined directly by electron microscopy. Its appearance was that of duplex DNA. In addition, some duplex DNA was observed under conditions in which the sedimentation anomaly was no longer apparent. Persistence of double-stranded DNA under denaturing conditions suggests caution in the interpretation of changes in the molecular weight or conformation of DNA based solely on analysis of sedimentation profiles.

MeSH Terms
Animals Cell Line Centrifugation, Density Gradient Cricetinae DNA/biosynthesis,isolation & purification Fibroblasts Hydrogen-Ion Concentration Lung Microscopy, Electron Nitrogen Mustard Compounds Nucleic Acid Conformation Nucleic Acid Denaturation Thymidine/metabolism Tritium
Chemicals
Nitrogen Mustard Compounds Tritium DNA Thymidine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Simpson J R
Nagle W A
Bick M D
Belli J A
References (35)
35 references, click to expand
  1. Mechanism of inactivation of DNA and RNA bacteriophages by alkylating agents in vitro.
    Cancer Res. 1966 Nov;26(11):2301-6 PMID: 5956634
  2. DNA fibres from human lymphocyte nuclei.
    Exp Cell Res. 1966 Nov-Dec;44(2):642-5 PMID: 5337209
  3. Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces.
    Nature. 1966 Oct 29;212(5061):534-5 PMID: 5339143
  4. Interstrand cross-linking of DNA by difunctional alkylating agents.
    J Mol Biol. 1967 Apr 14;25(1):143-60 PMID: 5340530
  5. Rejoining of x-ray induced breaks in the DNA of leukaemia cells.
    Nature. 1967 May 20;214(5090):790-2 PMID: 6051854
  6. Characterization of a naturally occurring, cross-linked fraction of DNA. 1. Nature of the cross-linkage.
    J Mol Biol. 1968 Mar 14;32(2):379-403 PMID: 4966870
  7. Characterization of a naturally occurring, cross-linked fraction of DNA. II. Origin of the cross-linkage.
    J Mol Biol. 1968 Mar 14;32(2):405-21 PMID: 4966871
  8. The rationale for an ordered arrangement of chromatin in the interphase nucleus.
    Am J Hum Genet. 1968 Sep;20(5):440-60 PMID: 5701616
  9. Artefact in the measurement of the molecular weight of pulse labelled DNA.
    Nature. 1969 Mar 15;221(5185):1053-6 PMID: 5774397
  10. Radiation-induced breaks of DNA in cultured mammalian cells.
    Biophys J. 1969 Mar;9(3):330-46 PMID: 5780712
  11. DNA-strand breaks and rejoining following exposure of synchronized Chinese hamster cells to ionizing radiations.
    Mutat Res. 1968 Nov-Dec;6(3):459-65 PMID: 5728836
  12. Potentially lethal radiation damage: repair by mammalian cells in culture.
    Science. 1969 Aug 1;165(3892):490-2 PMID: 5793240
  13. Effects of the conformation of single-stranded DNA on renaturation and aggregation.
    J Mol Biol. 1969 Apr;41(2):199-209 PMID: 5801474
  14. Two forms of repair of DNA in mammalian cells following irradiation.
    Biophys J. 1970 Mar;10(3):237-45 PMID: 5461576
  15. On the size of the DNA in the mammalian chromosome. Structural subunits.
    Biophys J. 1970 Mar;10(3):277-92 PMID: 5265017
  16. An improvement in alkaline sucrose density gradient sedimentation of mammalian cell DNA.
    Radiat Res. 1970 Dec;44(3):713-26 PMID: 5489167
  17. The production of strand breaks in mammalian DNA by X-rays: at different stages in the cell cycle.
    Radiat Res. 1970 Dec;44(3):771-87 PMID: 5530792
  18. The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex.
    Proc Natl Acad Sci U S A. 1971 Jan;68(1):6-9 PMID: 4924971
  19. The release of high molecular weight DNA from a mammalian cell (L-5178Y). Attachment of the DNA to the nuclear membrane.
    Biochim Biophys Acta. 1971 Jan 28;228(2):331-43 PMID: 5101928
  20. Sedimentation of DNA released from Chinese hamster cells.
    Biophys J. 1971 Jun;11(6):502-20 PMID: 4997648
  21. Daughter cell repair by mammalian cells in culture after potentially lethal radiation damage.
    Nat New Biol. 1971 Sep 8;233(36):47-8 PMID: 5286355
  22. Anomalous sedimentation of high molecular weight denatured mammalian DNA.
    Biochem Biophys Res Commun. 1971 Jul 2;44(1):171-7 PMID: 5116507
  23. DNA damage and repair following irradiation: the effect of 5-bromodeoxyuridine in cultured Chinese hamster cells.
    Radiat Res. 1971 Oct;48(1):86-94 PMID: 5165688
  24. Sedimentation characteristics of DNA multiply crosslinked by a difunctional alkylating agent, mustard gas.
    Biopolymers. 1971 Nov;10(11):2079-82 PMID: 5118644
  25. Artefacts arising from the sedimentation of high molecular weight DNA on sucrose gradients.
    Biochim Biophys Acta. 1971 Oct;247(3):369-72 PMID: 5166496
  26. Sedimentation analysis of DNA from irradiated and unirradiated L-cells.
    Biophys J. 1972 Apr;12(4):369-83 PMID: 5063052
  27. Modification of chromatin by trypsin. The role of proteins in maintainance of deoxyribonucleic acid conformation.
    Biochemistry. 1972 May 23;11(11):2003-8 PMID: 5027613
  28. Sedimentation properties of mammalian-cell DNA: evidence that non-specific molecular aggregation does not occur during cell lysis.
    Int J Radiat Biol Relat Stud Phys Chem Med. 1972 Jun;21(6):603-6 PMID: 4537776
  29. On the structure of the folded chromosome of Escherichia coli.
    J Mol Biol. 1972 Nov 14;71(2):127-47 PMID: 4564477
  30. Radiation induced strand breakage in DNA from mammalian cells. Strand separation in alkaline solution.
    Int J Radiat Biol Relat Stud Phys Chem Med. 1973 Mar;23(3):285-9 PMID: 4633439
  31. Repair of DNA containing interstrand crosslinks in Escherichia coli: sequential excision and recombination.
    Proc Natl Acad Sci U S A. 1973 Apr;70(4):1064-8 PMID: 4577788
  32. Repair of a DNA complex from x-irradiated Chinese hamster cells.
    Int J Radiat Biol Relat Stud Phys Chem Med. 1972 Jul;22(1):75-90 PMID: 4541706
  33. "Reversible" DNA.
    Proc Natl Acad Sci U S A. 1961 Jul 15;47:950-5 PMID: 13704192
  34. EXPERIMENTAL CHEMOTHERAPY STUDIES. 3. PROPERTIES OF DNA FROM ASCITES CELLS TREATED IN VIVO WITH NITROGEN MUSTARD.
    Cancer Res. 1964 Jul;24:964-8 PMID: 14198245
  35. MOLECULAR SIZE AND CIRCULARITY OF DNA IN CELLS OF MAMMALS AND HIGHER PLANTS.
    Proc Natl Acad Sci U S A. 1965 Feb;53:356-62 PMID: 14294069
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.12.3660
Published
1973-12-00
Pages
3660-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427301
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.12.3660 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2