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PMID: 4521823 Published · ppublish English Journal Article

Identification of discrete polyadenylate-containing RNA components transcribed from HeLa cell mitochondrial DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 2 ·1974-02-00 ·Pages 563-7

Ojala D, Attardi G

Abstract

Polyacrylamide gel electrophoresis and sedimentation analysis under denaturing conditions of poly(A)-containing RNA from the polysome region of the sedimentation pattern of a HeLa-cell mitochondrial lysate has revealed the occurrence of a discrete RNA component, which sediments in the native state with a sedimentation constant of about 7 S. From the sedimentation behavior under native and denaturing conditions and the poly(A) content, a molecular weight of about 9 x 10(4) has been estimated for this component. RNA.DNA hybridization experiments have indicated that this component is coded for by the light strand of mitochondrial DNA. Evidence for the occurrence of a poly(A)-containing RNA component sedimenting at about 9 S and coded for by the heavy strand has also been obtained.

MeSH Terms
Adenine Nucleotides/analysis Adenosine/metabolism Carbon Radioisotopes Centrifugation, Density Gradient DNA, Mitochondrial/metabolism DNA, Neoplasm/metabolism Dactinomycin/pharmacology Electrophoresis, Polyacrylamide Gel Female HeLa Cells/metabolism Humans Molecular Weight Nucleic Acid Denaturation Nucleic Acid Hybridization Polynucleotides/analysis RNA, Neoplasm/analysis,biosynthesis Transcription, Genetic Tritium Uridine/metabolism
Chemicals
Adenine Nucleotides Carbon Radioisotopes DNA, Mitochondrial DNA, Neoplasm Polynucleotides RNA, Neoplasm Tritium Dactinomycin Adenosine Uridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ojala D
Attardi G
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.2.563
Published
1974-02-00
Pages
563-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388048
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.2.563 PMC Full Text View journal details
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