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PMID: 4524648 Published · ppublish English Journal Article

Protein fusion: a novel reaction in bacteriophage lambda head assembly.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 4 ·1974-04-00 ·Pages 1451-5

Hendrix RW, Casjens SR

Abstract

Parts of two phage-coded head proteins, pE and pC, become fused during bacteriophage lambda head assembly. pE is the main structural component of lambda heads and pC is a minor head protein that is not found as such in mature heads. The bond joining the two proteins appears to be covalent and is not a disulfide bond. Only a specific subset of the sequences of each protein is found in the fusion products, and these sequences are found in the products in equimolar amounts. Two nearly identical fusion products; X1 and X2, are detected; X2 is slightly smaller than X1 and appears to be a proteolytic cleavage product of X1. The fusion reaction probably takes place on a nascent head structure.

MeSH Terms
Autoradiography Chromosome Mapping Coliphages/analysis,growth & development,metabolism DNA Viruses/analysis,growth & development,metabolism Electrophoresis, Polyacrylamide Gel Genotype Molecular Weight Morphogenesis Protein Binding Sulfur Radioisotopes Viral Proteins/analysis,biosynthesis
Chemicals
Sulfur Radioisotopes Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hendrix R W
Casjens S R
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.4.1451
Published
1974-04-00
Pages
1451-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388247
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.4.1451 PMC Full Text View journal details
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