A functional mitotic spindle prepared from mammalian cells in culture.
MITOTIC CELLS LYSED INTO SOLUTIONS OF POLYMERIZABLE MICROTUBULE PROTEIN CONTAIN A SPINDLE WHICH IS SIMILAR TO THE LIVING SPINDLE IN TWO RESPECTS: it will lose and gain birefringence when cooled and warmed, and it will move anaphase chromosomes to the opposite ends of the cell. Early anaphase cells lysed into buffers containing high molecular weight polyethylene glycol and nucleotide triphosphates will continue chromosome motion and spindle elongation in the absence of exogenous spindle subunits. These results suggest that while spindle growth requires microtubule polymerization, anaphase motions do not.
18 references, click to expand
-
Nucleated assembly of microtubules in porcine brain extracts.Science. 1972 Sep 29;177(4055):1196-7 PMID: 5057625
-
Procedure for embedding in situ selected cells cultured in vitro.J Cell Biol. 1967 Oct;35(1):279-83 PMID: 4863049
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
-
The effects of colcemid inhibition and reversal on the fine structure of the mitotic apparatus of Chinese hamster cells in vitro.J Ultrastruct Res. 1967 Jul;19(1):1-18 PMID: 5339063
-
An actin-like component in spermatocytes of a crane fly (Nephrotoma suturalis Loew). I. The spindle.Chromosoma. 1972;39(2):145-73 PMID: 4343921
-
The mitotic apparatus. Fine structure of the isolated unit.J Cell Biol. 1962 Nov;15:279-87 PMID: 13962188
-
Microtubule formation in vitro in solutions containing low calcium concentrations.Science. 1972 Sep 22;177(4054):1104-5 PMID: 4626639
-
Cell motility by labile association of molecules. The nature of mitotic spindle fibers and their role in chromosome movement.J Gen Physiol. 1967 Jul;50(6):Suppl:259-92 PMID: 6058222
-
Flagellar movement and adenosine triphosphatase activity in sea urchin sperm extracted with triton X-100.J Cell Biol. 1972 Jul;54(1):75-97 PMID: 4261039
-
An estimate of the amount of microtubule protein in the isolated mitotic apparatus.J Cell Sci. 1970 Jan;6(1):159-76 PMID: 5461428
-
C-microtubules in isolated mitotic spindles.J Cell Sci. 1971 Nov;9(3):603-19 PMID: 5169780
-
[Adenosinetriphosphate as the energy substance for cell movement].Biochim Biophys Acta. 1954 Jun;14(2):182-94 PMID: 13172235
-
A Ca-activated ATPase in the mitotic apparatus of the sea urchin egg (isolated by a new method).Exp Cell Res. 1972 Feb;70(2):325-32 PMID: 4258131
-
The structure and some properties of the isolated mitotic apparatus.J Cell Sci. 1969 Jan;4(1):179-209 PMID: 4180667
-
The mechanism of action of colchicine. Colchicine binding to sea urchin eggs and the mitotic apparatus.J Cell Biol. 1967 Aug;34(2):535-48 PMID: 6035643
-
Protein measurement with the Folin phenol reagent.J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
-
Immunochemical studies of 22S protein from isolated mitotic apparatus.J Cell Biol. 1969 May;41(2):577-90 PMID: 4977446
-
Ultrastructure and birefringence of the isolated mitotic apparatus of marine eggs.J Cell Biol. 1967 Sep;34(3):859-83 PMID: 6057812
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