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PMID: 4525299 Published · ppublish English Journal Article

Characterization of a protein species isolated from HeLa cell cytoplasm by affinity chromatography on polyadenylate-sepharose.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 5 ·1974-05-00 ·Pages 1882-6

Blanchard JM, Brissac C, Jeanteur P

Abstract

Chromatography of different soluble extracts from HeLa cells on poly(A)-Sepharose columns has allowed the isolation of a protein fraction eluted by 0.2 M NaCl and localized predominantly in the cytoplasmic supernatant and in the 0.5 M KCl ribosomal wash. This fraction is present in large amounts (around 3% of total cytosolic proteins) and appears to contain a major protein species that is acidic on electrofocusing (pI around 4.5) and phosphorylated. It runs on glycerol gradients and Sephadex G-200 chromatography close to the aldolase marker (158,000 daltons) and dissociates into apparently identical subunits of 38,000 +/- 2,000 daltons on sodium dodecyl sulfate-acrylamide gels, suggesting a tetrameric structure.

MeSH Terms
Amino Acids Centrifugation, Density Gradient Chemical Phenomena Chemistry Chromatography Chromatography, Gel Electrophoresis Electrophoresis, Polyacrylamide Gel Female Glycerol HeLa Cells/analysis Humans Isoelectric Focusing Kinetics Neoplasm Proteins/isolation & purification Oligonucleotides Polysaccharides Sodium Dodecyl Sulfate
Chemicals
Amino Acids Neoplasm Proteins Oligonucleotides Polysaccharides Sodium Dodecyl Sulfate Glycerol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blanchard J M
Brissac C
Jeanteur P
References (35)
35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.5.1882
Published
1974-05-00
Pages
1882-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388346
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.5.1882 PMC Full Text View journal details
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