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PMID: 4530298 Published · ppublish English Journal Article

Cholera toxin and cell growth: role of membrane gangliosides.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 10 ·1974-10-00 ·Pages 4224-8

Hollenberg MD, Fishman PH, Bennett V, Cuatrecasas P

Abstract

The binding of cholera toxin to three transformed mouse cell lines derived from the same parent strain, and the effects of the toxin on DNA synthesis and adenylate cyclase activity, vary in parallel with the ganglioside composition of the cells. TAL/N cells of early passage, which contain large quantities of gangliosides G(M3), G(M2), G(M1), and G(Dla), as well as the glycosyltransferases necessary for the synthesis of these gangliosides, bind the most cholera toxin and are the most sensitive to its action. TAL/N cells of later passage, which lack chemically detectable G(M1) and G(Dla) and which have no UDP-Gal:G(M2) galactosyltransferase activity, are intermediate in binding and response to the toxin. SVS AL/N cells, which lack G(M2) in addition to G(M1) and G(Dla) and which have little detectable UDP-GalNAc:G(M3)N-acetylgalactosaminyltransferase activity, bind the least amount of toxin. The SVS AL/N cells are the least responsive to inhibition of DNA synthesis and stimulation of adenylate cyclase activity by cholera toxin. Gangliosides (especially G(M1)), which appear to be the natural membrane receptors for cholera toxin, may normally have important roles in the regulation of cell growth and cAMP-mediated responses.

MeSH Terms
Adenylyl Cyclases/metabolism Animals Cell Division Cell Line Cell Membrane/metabolism Cell Transformation, Neoplastic DNA/biosynthesis DNA, Neoplasm/biosynthesis Endotoxins/metabolism,pharmacology Gangliosides/metabolism Hexosyltransferases/metabolism Iodine Radioisotopes Mice Receptors, Drug Vibrio cholerae
Chemicals
DNA, Neoplasm Endotoxins Gangliosides Iodine Radioisotopes Receptors, Drug DNA Hexosyltransferases Adenylyl Cyclases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hollenberg M D
Fishman P H
Bennett V
Cuatrecasas P
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50 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.10.4224
Published
1974-10-00
Pages
4224-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC434363
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.10.4224 PMC Full Text View journal details
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