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PMID: 4541123 Published · ppublish English Journal Article

Regulation of the immune response. 3. Kinetic differences between thymus- and bone marrow- derived lymphocytes in the proliferative response to heterologous erythrocytes.

The Journal of experimental medicine ·Vol. 137 ·No. 6 ·1973-06-01 ·Pages 1325-37

Kappler JW, Hoffmann M

Abstract

The kinetics of the in vivo response to SRBC was studied in mouse spleen at both the B cell and T cell levels. The B cell response was assayed by following the appearance of antibody-secreting cells in the spleen using the hemolytic plaque assay. The T cell response was monitored by following the increase in or "priming" of helper activity in the spleen using a quantitative in vitro assay. The role of cellular proliferation in both responses was established with the inhibitor of mitosis, vinblastine. The results show that, although the development of T cell activity precedes that of anti-SRBC PFC by as much as 1 day, T cells lag at least 1 day behind B cells in the onset of cellular proliferation. The evidence suggests either that the helper T cell which proliferates in response to SRBC does so after helping in the initiation of the primary B cell response or that the proliferative T cell response and the initiation of the primary B cell response involve two different subpopulations of T cells.

MeSH Terms
Animals Antibody Formation Antigens Bone Marrow/immunology Bone Marrow Cells Cell Division Cells, Cultured Erythrocyte Transfusion Erythrocytes/immunology Kinetics Mice Sheep/immunology T-Lymphocytes/drug effects,immunology Vinblastine/pharmacology Viral Plaque Assay
Chemicals
Antigens Vinblastine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kappler J W
Hoffmann M
References (31)
31 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.137.6.1325
Published
1973-06-01
Pages
1325-37
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139343
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.137.6.1325 PMC Full Text View journal details
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