Differential metabolism of large and small poly(A) sequences in the heterogeneous nuclear RNA of HeLa cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 1 ·1974-01-00 ·Pages 200-4

Nakazato H, Edmonds M, Kopp DW

Abstract

The heterogeneous RNA of the HeLa cell nucleus contains short internal poly(A) sequences which, in contrast to the longer poly(A) sequences at the 3' ends, are not found in messenger RNA of the cytoplasm. A distinct origin for each of these homologous sequences is evident when the effects of actinomycin D and 3'-deoxyadenosine on their biosynthesis are compared. The shorter poly(A) appears to be transcribed, while the longer one does not. A kinetic analysis of the metabolism of each type of poly(A) sequence within different size classes of HnRNA after treatment of the cells with 3'-deoxyadenosine reveals distinctive distribution and metabolism of these sequences and also provides insight into mechanisms proposed for the generation of messenger RNA from these large RNA molecules in the nucleus.

MeSH Terms

Adenine Nucleotides/analysis,biosynthesis,metabolism Adenosine/metabolism Base Sequence Cell Nucleus/metabolism Dactinomycin/pharmacology Deoxyadenosines/pharmacology Electrophoresis, Polyacrylamide Gel Female HeLa Cells/metabolism Humans Kinetics Molecular Weight Phosphorus Radioisotopes Polynucleotides/analysis,biosynthesis,metabolism RNA, Messenger/biosynthesis RNA, Neoplasm/analysis,metabolism Transcription, Genetic Tritium

Chemicals

Adenine Nucleotides Deoxyadenosines Phosphorus Radioisotopes Polynucleotides RNA, Messenger RNA, Neoplasm Tritium Dactinomycin Adenosine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Nakazato H

Edmonds M

Kopp D W

References (15)

15 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.1.200

Published

1974-01-00

Pages

200-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC387965

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.1.200 PMC Full Text View journal details

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Differential metabolism of large and small poly(A) sequences in the heterogeneous nuclear RNA of HeLa cells.

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