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PMID: 4547843 Published · ppublish English Journal Article

Elongation of the polyadenylate segment of messenger RNA in the cytoplasm of mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 10 ·1974-10-00 ·Pages 4091-5

Diez J, Brawerman G

Abstract

Chinese hamster and mouse sarcoma 180 ascites cells, treated with high levels of actinomycin D, still carry out limited poly(A) synthesis. The residual activity, which consists of poly(A) chain extension in the cytoplasm as well as in the nucleus, is essentially insensitive to cordycepin. Nuclear polyadenylation proceeds linearly and involves the gradual extension of unusually long poly(A) sequences. Cytoplasmic poly(A) labeling is initially more rapid than in the nucleus, but levels off within 5-10 min. It consists of addition of seven to eight AMP residues to the poly(A) sequences in preexisting mRNA molecules. The levelling off can be accounted for by a rapid turnover of the extremity of the poly(A) chain in the mRNA. Cytoplasmic poly(A) chain extension can be detected in cells not subjected to the actinomycin treatment. The rate of this process is of the same order of magnitude as that of new poly(A) transfer from nucleus to cytoplasm. It could serve to control the length of the poly(A) sequence in mRNA.

MeSH Terms
Adenine Nucleotides/biosynthesis Adenosine/metabolism Animals Cell Line Cell Nucleus/metabolism Centrifugation, Density Gradient Cricetinae Cytoplasm/metabolism Dactinomycin/pharmacology Deoxyadenosines/pharmacology Depression, Chemical Mice Polynucleotides/biosynthesis Polyribosomes/metabolism RNA, Messenger/analysis,biosynthesis RNA, Neoplasm/biosynthesis Sarcoma 180 Tritium
Chemicals
Adenine Nucleotides Deoxyadenosines Polynucleotides RNA, Messenger RNA, Neoplasm Tritium Dactinomycin Adenosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Diez J
Brawerman G
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17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.10.4091
Published
1974-10-00
Pages
4091-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC434334
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.10.4091 PMC Full Text View journal details
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