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PMID: 4558663 Published · ppublish English Journal Article

Deletion mutants of bacteriophage phiX174.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 7 ·1972-07-00 ·Pages 1905-10

Zuccarelli AJ, Benbow RM, Sinsheimer RL

Abstract

Mutants of bacteriophage varphiX174 have been isolated that are less dense than wild-type varphiX particles in CsCl. When mutant viral (+) strand DNA and wild-type complementary (-) strand DNA are hybridized, the resulting duplex molecules have single-stranded loops characteristic of wild-type-deletion heteroduplexes. The mutant bacteriophages fail to complement varphiX amber mutants in cistron E. We conclude that the mutant viruses have deleted approximately 7% of the varphiX genome in the region of cistron E.

MeSH Terms
Carbon Isotopes Centrifugation, Density Gradient Coliphages DNA, Single-Stranded DNA, Viral Electrophoresis, Disc Escherichia coli/drug effects Genetics, Microbial Helper Viruses Mathematics Microscopy, Electron Mitomycins/pharmacology Mutation Nucleic Acid Hybridization Tritium Virus Replication
Chemicals
Carbon Isotopes DNA, Single-Stranded DNA, Viral Mitomycins Tritium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zuccarelli A J
Benbow R M
Sinsheimer R L
References (23)
23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.7.1905
Published
1972-07-00
Pages
1905-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426829
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.7.1905 PMC Full Text View journal details
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