Home LiteratureArticle Details
PMID: 4563978 Published · ppublish English Journal Article

Deletion and complementation analysis of biotin gene cluster of Escherichia coli.

Journal of bacteriology ·Vol. 112 ·No. 2 ·1972-11-00 ·Pages 830-9

Cleary PP, Campbell A

Abstract

Genetic deletions that terminate within the cluster of genes needed for biotin biosynthesis in Escherichia coli have been isolated and mapped by transduction with phages lambda and P1. These deletions order the point mutations in each of the five genes. Mutations causing biotin dependence were incorporated into lambdapbio transducing phages. New bio(-) mutations were induced by exposure of lambdapbio particles to ultraviolet light. Tests of complementation between such bio(-)pbio particles and bio(-) mutant cells divide the bio(-) mutations into five cistrons: bioA, bioB, bioF, bioC, and bioD. Certain bioA and bioF mutations exhibit intragenic complementation, suggesting that these genes determine enzymes composed of identical subunits.

MeSH Terms
Biotin/biosynthesis Chlorates/pharmacology Coliphages Drug Resistance, Microbial Escherichia coli/drug effects,growth & development,metabolism,radiation effects Genes Genetic Complementation Test Lysogeny Mutation Radiation Effects Transduction, Genetic Ultraviolet Rays
Chemicals
Chlorates Biotin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cleary P P
Campbell A
References (31)
31 references, click to expand
  1. Synthesis of 7-oxo-8-aminopelargonic acid, a biotin vitamer, in cell-free extracts of Escherichia coli biotin auxotrophs.
    J Bacteriol. 1968 Oct;96(4):1291-7 PMID: 4879561
  2. Current linkage map of Escherichia coli.
    Bacteriol Rev. 1970 Jun;34(2):155-75 PMID: 4918631
  3. Divergent orientation of transcription from the biotin locus of Escherichia coli.
    J Mol Biol. 1971 Feb 28;56(1):53-62 PMID: 4929887
  4. A deletion analysis of prophage lambda and adjacent genetic regions.
    Proc Natl Acad Sci U S A. 1968 Nov;61(3):956-62 PMID: 5246560
  5. Specialized transduction of galactose by lambda phage from a deletion lysogen.
    Virology. 1970 Jul;41(3):474-87 PMID: 4912822
  6. The galactose operon of E. coli K-12. II. A deletion analysis of operon structure and polarity.
    Genetics. 1969 Jun;62(2):249-64 PMID: 4905872
  7. Studies on the genetics of biotin-transducing, defective variants of bacteriophage lambda.
    Virology. 1968 Sep;36(1):30-41 PMID: 5669988
  8. Nonessential functions of bacteriophage lambda.
    Virology. 1969 Feb;37(2):177-88 PMID: 4974326
  9. On the production of deletions in the chromosome of Escherichia coli.
    J Mol Biol. 1970 Oct 14;53(1):49-67 PMID: 4922221
  10. Histidine regulatory mutants in Salmonella typhimurium. IV. A positive selection for polar histidine-requiring mutants from histidine operator constitutive mutants.
    J Mol Biol. 1967 Nov 28;30(1):81-95 PMID: 4294871
  11. Biotin-requiring mutants of Escherichia coli K-12.
    J Bacteriol. 1967 Dec;94(6):2065-6 PMID: 4864413
  12. Biosynthesis of desthiobiotin in cell-free extracts of Escherichia coli.
    J Bacteriol. 1969 Sep;99(3):696-701 PMID: 4905533
  13. Lambda phage transduction of the bio A locus of Escherichia coli.
    Virology. 1970 Nov;42(3):643-61 PMID: 4921685
  14. CHROMOSOMAL ABERRATIONS ASSOCIATED WITH MUTATIONS TO BACTERIOPHAGE RESISTANCE IN ESCHERICHIA COLI.
    J Bacteriol. 1965 Jan;89:28-40 PMID: 14255678
  15. The regulatory mechanism of enzyme synthesis in the tryptophan biosynthetic pathway of Escherichia coli K-12.
    Biochem Biophys Res Commun. 1962 Oct 17;9:204-7 PMID: 13933809
  16. THE BIOSYNTHESIS OF BIOTIN IN MICROORGANISMS. I. THE PHYSIOLOGY OF BIOTIN SYNTHESIS IN ESCHERICHIA COLI.
    Biochim Biophys Acta. 1965 Apr 12;100:28-35 PMID: 14323635
  17. Immunity phase-shift in defective lysogens: non-mutational hereditary change of early regulation of lambda prophage.
    J Mol Biol. 1970 Jul 14;51(1):1-13 PMID: 4921249
  18. Sensitive mutants of bacteriophage lambda.
    Virology. 1961 May;14:22-32 PMID: 13690195
  19. Biosynthesis of biotin in microorganisms. VI. Further evidence for desthiobiotin as a precursor in Eschericia coli.
    J Bacteriol. 1967 Dec;94(6):1930-3 PMID: 4864407
  20. Genetic and biochemical analysis of the isoenzymes concerned in the first reaction of aromatic biosynthesis in Escherichia coli.
    J Bacteriol. 1967 Jan;93(1):237-44 PMID: 5335890
  21. Mechanism of biotin action.
    Annu Rev Biochem. 1970;39:757-76 PMID: 4920829
  22. Evidence for a prophage excision gene in lambda.
    J Mol Biol. 1970 Feb 14;47(3):557-64 PMID: 4907271
  23. Integration-negative mutants of bacteriophage lambda.
    J Mol Biol. 1968 Feb 14;31(3):487-505 PMID: 5637199
  24. New controlling element in the Lac operon of E. coli.
    Nature. 1968 Mar 2;217(5131):825-7 PMID: 4867974
  25. Deletion-mapping of resistance against chlorate in Klebsiella aerogenes.
    Mol Gen Genet. 1970;106(2):174-9 PMID: 5415254
  26. Frameshift mutations and the genetic code. This paper is dedicated to Professor Theodosius Dobzhansky on the occasion of his 66th birthday.
    Cold Spring Harb Symp Quant Biol. 1966;31:77-84 PMID: 5237214
  27. An early intermediate in the biosynthesis of biotin: Incorporation studies with [1,7-C(2)]pimelic acid.
    Biochem J. 1966 Dec;101(3):601-6 PMID: 16742432
  28. The purification and properties of dethiobiotin synthetase.
    J Biol Chem. 1970 Dec 25;245(24):6558-66 PMID: 4921568
  29. Genetic analysis of the maltose A region in Escherichia coli.
    J Bacteriol. 1969 May;98(2):559-67 PMID: 4891257
  30. Genetic and biochemical analysis of the biotin loci of Escherichia coli K-12.
    J Bacteriol. 1968 Aug;96(2):515-24 PMID: 4877129
  31. Studies on radiation-sensitive mutants of E. coli. I. Mutants defective in the repair synthesis.
    Mol Gen Genet. 1968 May 3;101(3):227-44 PMID: 4879097
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.112.2.830-839.1972
Published
1972-11-00
Pages
830-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251493
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.112.2.830-839.1972 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2