A coat protein of the bacteriophage M13 virion participates in membrane-oriented synthesis of DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 1 ·1973-01-00 ·Pages 205-9

Jazwinski SM, Marco R, Kornberg A

Abstract

Several molecules of a protein specified by gene 3 of M13 comprise a minor fraction of the phage coat and have been assigned a role in adsorption to the bacterial cell. We find that the gene-3 protein molecules of the virion are fully conserved in phage that have attached irreversibly to the host cell, and they form a complex with the phage DNA when it has been converted to a duplex replicative form. In cells infected at a restrictive temperature with a thermosensitive mutant in gene 3, there is no conversion of the phage DNA to the replicative form. Both the adsorbed phage and the complex of replicative form DNA with the gene-3 protein were isolated with the inner membrane fraction of the cell. We suggest that the gene-3 protein may perform an essential function in the synthesis of replicative form by linking the phage DNA to a cellular replicative system in or on the inner cell membrane.

MeSH Terms

Cell Membrane Centrifugation, Density Gradient Coliphages/metabolism DNA Replication DNA, Viral/biosynthesis Electrophoresis, Polyacrylamide Gel Escherichia coli/cytology,growth & development Genes Genetic Code Histidine/metabolism Lysogeny Mutation Phosphates/metabolism Phosphorus Isotopes Temperature Thymine/metabolism Tritium Viral Proteins/analysis,biosynthesis,metabolism

Chemicals

DNA, Viral Phosphates Phosphorus Isotopes Viral Proteins Tritium Histidine Thymine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jazwinski S M

Marco R

Kornberg A

References (25)

25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.1.205

Published

1973-01-00

Pages

205-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433216

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.1.205 PMC Full Text View journal details

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A coat protein of the bacteriophage M13 virion participates in membrane-oriented synthesis of DNA.

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