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PMID: 4561204 Published · ppublish English Journal Article

Filamentous bacterial viruses. V. Asymmetric replication of fd duplex deoxyribonucleic acid.

Journal of virology ·Vol. 10 ·No. 3 ·1972-09-00 ·Pages 371-83

Tseng BY, Marvin DA

Abstract

Short pulses (30 sec at 32 C) of (3)H-thymidine were found primarily in the viral strands of replicating fd deoxyribonucleic acid (DNA), even at a time when most DNA being synthesized was duplex DNA. Much of the labeled viral strand DNA was longer than unit length, but some was shorter than unit length. Most of the corresponding complementary-strand DNA was recovered in closed supercoiled duplex molecules, even for short pulses; the remainder of the complementary-strand DNA was found in replicative intermediates in pieces shorter than unit length. Some of the viral strands in open replicating DNA lacked a corresponding complementary strand.

MeSH Terms
Centrifugation, Density Gradient Coliphages/growth & development,metabolism DNA Replication DNA, Bacterial/analysis,biosynthesis,isolation & purification DNA, Viral/analysis,biosynthesis,isolation & purification Escherichia coli/analysis,drug effects,metabolism Mitomycins/pharmacology Phosphoric Acids/metabolism Phosphorus Isotopes Thymidine/metabolism Thymine/metabolism Tritium
Chemicals
DNA, Bacterial DNA, Viral Mitomycins Phosphoric Acids Phosphorus Isotopes Tritium Thymine Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tseng B Y
Marvin D A
References (35)
35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.10.3.371-383.1972
Published
1972-09-00
Pages
371-83
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356476
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.10.3.371-383.1972 PMC Full Text View journal details
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