Membrane antigens specific for human lymphoid cells in the dividing phase.

The Journal of experimental medicine ·Vol. 138 ·No. 1 ·1973-07-01 ·Pages 64-70

Thomas DB, Phillips B

Abstract

Heteroantisera were raised in rabbits against human thymocytes or Burkitt's lymphoma EBI cells. After suitable absorption, the sera were specific for thymocytes and T lymphoblasts, or B lymphoblasts and several lymphoblastoid cell lines. The specificity of the antisera was confirmed by preparing purified populations of T or B lymphocytes on immunoabsorbent columns, followed by mitogenic stimulation with phytohemagglutinin. The antisera did not react with normal resting lymphocytes.

MeSH Terms

Adsorption Animals Antibodies Antigens Antigens, Neoplasm Autoradiography B-Lymphocytes/immunology Burkitt Lymphoma/immunology Cell Membrane/immunology Cells, Cultured Chromatography, Gel Fluorescent Antibody Technique Humans Immunoglobulin Fragments Lectins/pharmacology Lymphocyte Activation Lymphocytes/immunology Mitosis Palatine Tonsil/immunology Rabbits/immunology T-Lymphocytes/immunology Thymus Gland/immunology

Chemicals

Antibodies Antigens Antigens, Neoplasm Immunoglobulin Fragments Lectins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Thomas D B

Phillips B

References (11)

11 references, click to expand

Cellular electrophoretic mobility and the mitotic cycle.
J Gen Physiol. 1966 Mar;49(4):717-25 PMID: 5221375
Variable behaviour of blood group H on HeLa cell populations synchronized with thymidine.
Nature. 1968 Aug 31;219(5157):938-9 PMID: 5673008
Limited periods of gene expression in immunoglobulin-synthesizing cells.
Science. 1969 Jun 27;164(3887):1524-5 PMID: 4182344
Cyclic membrane changes in animal cells: transformed cells permanently display a surface architecture detected in normal cells only during mitosis.
Proc Natl Acad Sci U S A. 1971 Jan;68(1):244-7 PMID: 4322263
Expression of H-2 and Moloney leukemia virus-determined cell-surface antigens in synchronized cultures of a mouse cell line.
Proc Natl Acad Sci U S A. 1971 Mar;68(3):566-9 PMID: 5276763
ANTIGENIC PROPERTIES OF EXPERIMENTAL LEUKEMIAS. I. SEROLOGICAL STUDIES IN VITRO WITH SPONTANEOUS AND RADIATION-INDUCED LEUKEMIAS.
J Natl Cancer Inst. 1963 Oct;31:977-95 PMID: 14069836
Cyclic expression of blood group determinants in murine cells and their relationship to growth control.
Nature. 1971 Oct 1;233(5318):317-21 PMID: 4107314
An acute-leukemia antigen: correlation with clinical status.
N Engl J Med. 1972 Dec 21;287(25):1272-4 PMID: 4508573
Specific purification of lymphocyte populations on a digestible immunoabsorbent.
J Immunol. 1973 Jan;110(1):313-9 PMID: 4568186
Evidence for transformation of human B lymphocytes by PHA.
Nat New Biol. 1973 Feb 21;241(112):254-6 PMID: 4121653
Structural alterations in the surface membrane during the cell cycle.
J Cell Biol. 1971 Aug;50(2):562-4 PMID: 4107022

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.138.1.64

Published

1973-07-01

Pages

64-70

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2180550

Subset

External Links

PubMed source DOI: 10.1084/jem.138.1.64 PMC Full Text View journal details

Analysis Services

Membrane antigens specific for human lymphoid cells in the dividing phase.

Contact

WeChat Official Account