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PMID: 4583026 Published · ppublish English Journal Article

A specific inhibitor of polypeptide-chain initiation in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 10 ·1973-10-00 ·Pages 2874-8

Lee-Huang S, Lee H, Ochoa S

Abstract

An inhibitor of polypeptide-chain initiation was isolated from E. coli cells. This protein inhibits formation of the 30S or 70S initiation complex with either fMet-tRNA(f) as initiator and AUG, MS2 RNA, or late T4 RNA as messenger, or acPhe-tRNA as initiator and poly(U) as messenger. Chain elongation, e.g., poly(U) translation at high Mg(2+) concentration, is not inhibited. The inhibitor is rendered ineffective when active aminoacylation of tRNA is taking place, e.g., during natural mRNA translation. This inhibitor is distinct from the so-called interference (i) factors, which interfere exclusively with the action of initiation factor 3. Since the new inhibitor can apparently be turned on and off, it may have a regulatory function in translation.

MeSH Terms
Bacterial Proteins/isolation & purification,pharmacology Carbon Radioisotopes Coliphages Electrophoresis, Polyacrylamide Gel Escherichia coli/analysis,cytology Hydrolysis Methionine Peptide Chain Elongation, Translational/drug effects Peptide Chain Initiation, Translational/drug effects Phenylalanine Poly U/metabolism Polynucleotides/metabolism Protein Biosynthesis/drug effects RNA, Transfer/metabolism RNA, Viral/metabolism Ribosomes/analysis
Chemicals
Bacterial Proteins Carbon Radioisotopes Polynucleotides RNA, Viral Poly U Phenylalanine RNA, Transfer Methionine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lee-Huang S
Lee H
Ochoa S
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.10.2874
Published
1973-10-00
Pages
2874-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427129
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.10.2874 PMC Full Text View journal details
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