Association of lysozyme with ribosomes and ribosome precursor particles.

Journal of bacteriology ·Vol. 116 ·No. 2 ·1973-11-00 ·Pages 1059-61

Dimmitt K, Sypherd PS

Abstract

Lysozyme, used to prepare osmotically fragile cells, combines with ribosomal subunits unless salt is present in excess of 50 mM. The binding of lysozyme to precursor ribonucleoprotein particles can result in their conversion to particles which sediment as mature ribosomes.

MeSH Terms

Centrifugation, Density Gradient Escherichia coli/cytology,enzymology Iodine Radioisotopes Muramidase/metabolism Nucleoproteins/metabolism Osmotic Fragility Ribosomes/enzymology

Chemicals

Iodine Radioisotopes Nucleoproteins Muramidase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Dimmitt K

Sypherd P S

References (5)

5 references, click to expand

Interaction of ribosomes and the cell envelope of Escherichia coli mediated by lysozyme.
J Bacteriol. 1970 Feb;101(2):584-91 PMID: 4905312
Efficient trace-labelling of proteins with iodine.
Nature. 1958 Jul 5;182(4627):53 PMID: 13566175
Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis.
Science. 1966 Sep 2;153(3740):1119-20 PMID: 5331371
Use of Brij lysis as a general method to prepare polyribosomes from Escherichia coli.
Biochim Biophys Acta. 1967 Dec 19;149(2):489-95 PMID: 4866435
Isolation of a cell membrane-DNA-nascent RNA complex from bacteria.
J Mol Biol. 1969 Feb 28;40(1):65-76 PMID: 4983147

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.116.2.1059-1061.1973

Published

1973-11-00

Pages

1059-61

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285492

Subset

External Links

PubMed source DOI: 10.1128/jb.116.2.1059-1061.1973 PMC Full Text View journal details

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Association of lysozyme with ribosomes and ribosome precursor particles.

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