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PMID: 4586777 Published · ppublish English Journal Article

Studies on the biosynthesis of alpha-putrescinylthymine in bacteriophage phi W-14-infected Pseudomonas acidovorans.

Journal of virology ·Vol. 12 ·No. 6 ·1973-12-00 ·Pages 1427-33

Kelln RA, Warren RA

Abstract

The alpha-putrescinylthymine (putThy) in bacteriophage phiW-14 DNA is synthesized at the mononucleotide level: it is labeled by uracil or deoxyuridine but not by thymidine, and it appears in the acid-soluble pool of infected cells before the onset of phage DNA synthesis. The methylene group at the C-5 position of the pyrimidine moiety of putThy is derived in vivo from a C(1) unit. Extracts of a phage infected thymidine auxotroph of the host, Pseudomonas acidovorans, apparently contain a phage-specific thymidylate synthetase and a phage-specific activity which forms 5-hydroxymethyl dUMP from N(5), N(10)-methylene-tetrahydrofolate and dUMP.

MeSH Terms
Bacteriophages/growth & development,metabolism Carbon Radioisotopes Cell-Free System Chromatography, Paper DNA Replication DNA, Viral/biosynthesis Escherichia coli/enzymology Formaldehyde/metabolism Pseudomonas/metabolism Putrescine/biosynthesis Pyrimidines/biosynthesis Serine/metabolism Spectrophotometry Tetrahydrofolates/metabolism Thymidine/metabolism Thymidylate Synthase/metabolism Thymine/biosynthesis Transferases/metabolism Tritium Uracil/metabolism
Chemicals
Carbon Radioisotopes DNA, Viral Pyrimidines Tetrahydrofolates Tritium Formaldehyde Serine Uracil Transferases Thymidylate Synthase Thymine Putrescine Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kelln R A
Warren R A
References (16)
16 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.12.6.1427-1433.1973
Published
1973-12-00
Pages
1427-33
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356784
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.12.6.1427-1433.1973 PMC Full Text View journal details
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