Suppression of a mutation in gene 3 of bacteriophage T7 (T7 endonuclease I) by mutations in phage and host polynucleotide ligase.

Journal of virology ·Vol. 13 ·No. 1 ·1974-01-00 ·Pages 226-9

Sadowski PD

Abstract

Bacteriophage T7 bearing amber mutations in both gene 1.3 (T7 DNA ligase) and gene 3 (T7 endonuclease I) are viable when grown in suppressor-negative, ligase-negative hosts. This is evidenced by a high plating efficiency and a large burst size compared to the single mutants. These findings may be explained by a limited destruction of cellular DNA by the double mutant.

MeSH Terms

Carbon Radioisotopes Centrifugation, Density Gradient Coliphages/enzymology,growth & development Crosses, Genetic DNA, Bacterial/metabolism Endonucleases/biosynthesis Escherichia coli/enzymology,metabolism Mutation Polynucleotide Ligases/biosynthesis Suppression, Genetic Thymidine/metabolism Tritium Virus Replication

Chemicals

Carbon Radioisotopes DNA, Bacterial Tritium Endonucleases Polynucleotide Ligases Thymidine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Sadowski P D

References (12)

12 references, click to expand

The genetics and physiology of bacteriophage T7.
Virology. 1969 Nov;39(3):562-74 PMID: 4902069
The structural gene for a T7 endonuclease essential for phage DNA synthesis.
Proc Natl Acad Sci U S A. 1970 Jan;65(1):242-8 PMID: 5263754
DNA ligase mutants of Escherichia coli.
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1580-7 PMID: 4922293
Bacteriophage T7 endonuclease. I. Properties of the enzyme purified from T7 phage-infected Escherichia coli B.
J Biol Chem. 1971 Jan 10;246(1):209-16 PMID: 5541763
Degradation of Escherichia coli B deoxyribonucleic acid after infection with deoxyribonucleic acid-defective amber mutants of bacteriophage T7.
J Virol. 1970 Aug;6(2):149-55 PMID: 4925772
An endodeoxyribonuclease induced after infection with phage T7 bearing amber mutations in genes 3, 5, and 6.
Can J Biochem. 1972 Sep;50(9):1016-23 PMID: 4561327
Deoxyribonucleic acid polymerase of bacteriophage T7.
J Biol Chem. 1971 Nov 25;246(22):6867-73 PMID: 4942327
A mutant of bacteriophage T7 deficient in polynucleotide ligase.
J Biol Chem. 1971 Nov 25;246(22):6874-9 PMID: 5001611
A DNA polymerase induced by bacteriophage T7.
Eur J Biochem. 1971 Dec 10;23(3):497-504 PMID: 4945114
Bacteriophage T7-induced endonuclease II. Purification and properties of the enzyme.
J Biol Chem. 1972 Jan 10;247(1):146-56 PMID: 4552921
Gene 6 exonuclease of bacteriophage T7. I. Purification and properties of the enzyme.
J Biol Chem. 1972 Jan 10;247(1):305-10 PMID: 4336366
Partial suppression of bacteriophage T4 ligase mutations by T4 endonuclease II deficiency: role of host ligase.
J Virol. 1971 Apr;7(4):534-6 PMID: 4939389

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.13.1.226-229.1974

Published

1974-01-00

Pages

226-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC355279

Subset

External Links

PubMed source DOI: 10.1128/JVI.13.1.226-229.1974 PMC Full Text View journal details

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Suppression of a mutation in gene 3 of bacteriophage T7 (T7 endonuclease I) by mutations in phage and host polynucleotide ligase.

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