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PMID: 4592691 Published · ppublish English Journal Article

Effect of 5-bromodeoxyuridine on chromatin transcription in confluent fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 2 ·1974-02-00 ·Pages 455-9

Hill BT, Tsuboi A, Baserga R

Abstract

3T6 mouse fibroblasts were grown in 5-bromodeoxyuridine (BrdU) so that approximately 20% of the thymine residues in DNA were replaced by BrdU. BrdU replacement caused an alteration in the relative incorporation of labeled nucleotide precursors into RNA. The RNA synthesized by cells grown in BrdU has a lower proportion of adenine and a higher guanine complement. This was shown for (a) nascent RNA made in vivo by confluent monolayers of cells in culture; (b) RNA synthesized in vitro on a chromatin template with either homologous or heterologous RNA polymerase; and (c) RNA synthesized in vitro on a DNA template with a highly purified RNA polymerase. The product was completely digested by RNase. The relative decrease in the incorporation of adenine into RNA was reserved when BrdU-treated cells were allowed to proliferate in BrdU-free medium.

MeSH Terms
Adenosine/metabolism Adenosine Monophosphate/metabolism Adenosine Triphosphate/metabolism Animals Bromodeoxyuridine/pharmacology Cell Line Chromatin/biosynthesis,metabolism Cytosine Nucleotides/metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology Fibroblasts Guanosine/metabolism Guanosine Triphosphate/metabolism Mice RNA/biosynthesis Templates, Genetic Transcription, Genetic/drug effects Tritium Uracil Nucleotides/metabolism Uridine/metabolism
Chemicals
Chromatin Cytosine Nucleotides Uracil Nucleotides Tritium Guanosine Adenosine Monophosphate RNA Guanosine Triphosphate Adenosine Triphosphate DNA-Directed RNA Polymerases Bromodeoxyuridine Adenosine Uridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hill B T
Tsuboi A
Baserga R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.2.455
Published
1974-02-00
Pages
455-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388025
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.2.455 PMC Full Text View journal details
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