New chromatographic form of phenylalanine transfer ribonucleic acid from Escherichia coli growing exponentially in a low-phosphate medium.

Journal of bacteriology ·Vol. 118 ·No. 1 ·1974-04-00 ·Pages 209-12

Mann MB, Huang PC

Abstract

Reversed-phase chromatography has been used to detect the presence of a new form of phenylalanyl-transfer ribonucleic acid (Phe-tRNA) from Escherichia coli growing exponentially in media containing low but nonlimiting levels of inorganic phosphate. The amount of this extra Phe-tRNA form is greatest in slowly growing cells (0.8 generations/h), and becomes negligible in media supporting a rapid growth rate (2.14 generations/h).

MeSH Terms

Carbon Radioisotopes Chromatography Culture Media Escherichia coli/analysis,growth & development,metabolism Glucose/metabolism Glycerol/metabolism Phenylalanine/metabolism Phosphates/metabolism RNA, Bacterial/analysis RNA, Transfer/analysis Spectrophotometry Succinates/metabolism Tritium

Chemicals

Carbon Radioisotopes Culture Media Phosphates RNA, Bacterial Succinates Tritium Phenylalanine RNA, Transfer Glucose Glycerol

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Mann M B

Huang P C

References (12)

12 references, click to expand

Isoaccepting +RNA's in mouse plasma cell tumors that synthesize different myeloma protein.
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Behavior of chloramphenicol-induced phenylalanine transfer ribonucleic acid during recovery from chloramphenicol treatment in Escherichia coli.
Biochemistry. 1973 Dec 18;12(26):5289-94 PMID: 4586515
Altered chromatographic properties of tRNA from chloramphenicol-treated Escherichia coli.
Biochem Biophys Res Commun. 1969 Oct 8;37(2):296-304 PMID: 4898698
Physiologically induced changes in the property of phenylalanine tRNA in Escherichia coli.
J Mol Biol. 1968 Nov 28;38(1):25-40 PMID: 4941472
An iron-dependent modification of several transfer RNA species in Escherichia coli.
J Mol Biol. 1969 Dec 28;46(3):581-4 PMID: 4904109
Identification of the cytokinin-active ribonucleosides in pure Escherichia coli tRNA species.
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1448-53 PMID: 4922291
The sequence of phenylalanine tRNA from E. coli.
FEBS Lett. 1969 Jun;3(4):275-278 PMID: 11947028
A new change in leucine transfer RNA observed in Escherichia coli infected with bacteriophage T2.
Proc Natl Acad Sci U S A. 1967 Apr;57(4):979-85 PMID: 5340587
Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.
Biochim Biophys Acta. 1971 Feb 11;228(3):770-4 PMID: 5572614
In vivo synthesis of tRNA Tyr 1 and tRNA Tyr 2 : differences in "early" and "late log" E. coli MRE 600.
Biochem Biophys Res Commun. 1972 Mar 24;46(6):2006-11 PMID: 4553153
Role modifications in tyrosine transfer RNA: a modified base affecting ribosome binding.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.118.1.209-212.1974

Published

1974-04-00

Pages

209-12

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC246659

Subset

External Links

PubMed source DOI: 10.1128/jb.118.1.209-212.1974 PMC Full Text View journal details

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New chromatographic form of phenylalanine transfer ribonucleic acid from Escherichia coli growing exponentially in a low-phosphate medium.

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