Mutant of Escherichia coli K-12 missing acetolactate synthase activity.

Journal of bacteriology ·Vol. 120 ·No. 1 ·1974-10-00 ·Pages 536-8

Guardiola J, De Felice M, Iaccarino M

Abstract

A mutant requiring isoleucine and valine for growth, because of the absence of acetolactate synthase activity, has been isolated. At least one of three different genes (ilvG, ilvB, ilvI) is required for the expression of acetolactate synthase activity, thus suggesting the presence of three different acetolactate synthase isoenzymes.

MeSH Terms

Chromosome Mapping Escherichia coli/enzymology,growth & development,metabolism Genes Isoenzymes/metabolism Isoleucine/metabolism Mutation Oxo-Acid-Lyases/biosynthesis,metabolism Phenotype Transduction, Genetic Valine/metabolism

Chemicals

Isoenzymes Isoleucine Oxo-Acid-Lyases Valine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Guardiola J

De Felice M

Iaccarino M

References (16)

16 references, click to expand

Genetic and biochemical analysis of the isoenzymes concerned in the first reaction of aromatic biosynthesis in Escherichia coli.
J Bacteriol. 1967 Jan;93(1):237-44 PMID: 5335890
Further evidence for two distinct acetolactate synthetases in Aerobacter aerogenes.
Biochim Biophys Acta. 1967 Jul 11;139(2):502-4 PMID: 6034688
Escherichia coli K-12 mutants altered in the transport of branched-chain amino acids.
J Bacteriol. 1971 Dec;108(3):1034-44 PMID: 4945181
Two forms of biosynthetic acetohydroxy acid synthetase in Salmonella typhimurium.
Biochem Biophys Res Commun. 1972 Jul 25;48(2):437-43 PMID: 4557731
Isoleucine and valine metabolism in Escherichia coli. XX. Multiple forms of acetohydroxy acid synthetase.
Biochem Biophys Res Commun. 1972 Jul 25;48(2):444-50 PMID: 4557732
Linkage map of Escherichia coli strain K-12.
Bacteriol Rev. 1972 Dec;36(4):504-24 PMID: 4568762
Isoleucine and valine metabolism in Escherichia coli. XXI. Mutations affecting derepression and valine resistance.
J Bacteriol. 1973 Apr;114(1):183-94 PMID: 4572708
Temperature-sensitive growth inhibition by valine in Salmonella typhimurium: alteration of one form of acetohydroxy acid synthetase.
J Bacteriol. 1973 Oct;116(1):98-106 PMID: 4583227
Escherichia coli K-12 mutants altered in the transport systems for oligo- and dipeptides.
J Bacteriol. 1973 Nov;116(2):751-6 PMID: 4126826
Multiplicity of isoleucine, leucine, and valine transport systems in Escherichia coli K-12.
J Bacteriol. 1974 Feb;117(2):382-92 PMID: 4590464
Mutations affecting the different transport systems for isoleucine, leucine, and valine in Escherichia coli K-12.
J Bacteriol. 1974 Feb;117(2):393-405 PMID: 4590465
REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES.
J Bacteriol. 1965 Mar;89:654-60 PMID: 14273640
REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. 3. MAP ORDER OF THE STRUCTURAL GENES AND OPERATOR GENES.
J Bacteriol. 1965 Mar;89:661-4 PMID: 14273641
REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. I. GENETIC DEREPRESSION OF ENZYME FORMATION.
J Bacteriol. 1964 Mar;87:566-73 PMID: 14127571
Selecting bacterial mutants by the penicillin method.
Science. 1960 Feb 26;131(3400):604-5 PMID: 13851300
Biosynthesis of valine and isoleucine. 2. Formation of alpha-acetolactate and alpha-aceto-alpha-hydroxybutyrate in Neurospora crassa and Escherichia coli.
J Biol Chem. 1960 Aug;235:2316-21 PMID: 13856271

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.120.1.536-538.1974

Published

1974-10-00

Pages

536-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC245796

Subset

External Links

PubMed source DOI: 10.1128/jb.120.1.536-538.1974 PMC Full Text View journal details

Analysis Services

Mutant of Escherichia coli K-12 missing acetolactate synthase activity.

Contact

WeChat Official Account