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PMID: 4626503 Published · ppublish English Journal Article

Minimal requirements for commitment to sporulation in Bacillus megaterium.

Journal of bacteriology ·Vol. 111 ·No. 2 ·1972-08-00 ·Pages 557-65

Greene RA, Slepecky RA

Abstract

Commitment to sporulation was examined by means of both endotrophic sporulation and rejuvenation experiments. In both cases, a point of commitment to the completion of sporulation occurred at stage II. With 2 x 10(8) cells/ml, the process by which commitment occurred required 0.5 mm Mg(2+), 10.0 mm phosphate, and an energy source (minimum of 5.0 mm acetate). For completion of sporulation and formation of normal, heat-resistant refractile spores, 0.3 mm Ca(2+) was required. Except for this Ca(2+) requirement, the completion of sporulation after commitment was independent of the nature of the surrounding medium. Some aspects of the utilization of these required substances were examined.

MeSH Terms
Acetates/metabolism Bacillus megaterium/growth & development,metabolism Buffers Calcium/metabolism Carbon Isotopes Culture Media Hot Temperature Hydrogen-Ion Concentration Magnesium/metabolism Phosphates/metabolism Spectrophotometry Spores/growth & development Spores, Bacterial/growth & development,metabolism
Chemicals
Acetates Buffers Carbon Isotopes Culture Media Phosphates Magnesium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Greene R A
Slepecky R A
References (33)
33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.111.2.557-565.1972
Published
1972-08-00
Pages
557-65
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251318
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.111.2.557-565.1972 PMC Full Text View journal details
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