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PMID: 4632442 Published · ppublish English Journal Article

Viral RNAs associated with ribosomes in Sindbis virus-infected HeLa cells.

Journal of virology ·Vol. 11 ·No. 3 ·1973-03-00 ·Pages 399-415

Rosemond H, Sreevalsan T

Abstract

Virus specific RNA ribosome complexes were isolated by sucrose density gradient centrifugation of cytoplasmic extracts from HeLa cells infected at 42 C with an RNA(+) mutant (ts2) of Sindbis virus. Viral RNA-ribosome complexes were accumulated by infected cells treated with sodium fluoride and cycloheximide. The RNA-ribosome complexes were characterized by (i) their sensitivity to the action of ribonuclease or ethylenediaminetetraacetic acid, (ii) their density in cesium chloride gradients, and (iii) presence of host ribosomes and viral RNAs. The viral RNAs were isolated and characterized. The results showed that two species of single-stranded RNAs (a 28s and 18 to 15s species) were associated with the complexes. Base composition analysis of the viral RNAs indicated that both species had a higher adenine content than the 42s or 26s forms of viral RNAs. The RNAs associated with the ribosome complexes were virus specific since they annealed with denatured double-stranded RNAs from the infected cells. Little or no 42S RNA was associated with the RNA-ribosome complexes. The results suggest that the 28s and 18 to 15s forms of RNAs may represent viral messenger RNAs.

MeSH Terms
Adenine/analysis Carbon Isotopes Centrifugation, Density Gradient Cycloheximide/pharmacology Edetic Acid Electrophoresis, Polyacrylamide Gel Fluorides/pharmacology HeLa Cells/drug effects,microbiology Humans Mutation Nucleic Acid Hybridization Phosphorus Isotopes RNA/isolation & purification RNA, Messenger/analysis RNA, Viral/analysis,isolation & purification Ribonucleases Ribosomes Sindbis Virus/growth & development,isolation & purification Tritium
Chemicals
Carbon Isotopes Phosphorus Isotopes RNA, Messenger RNA, Viral Tritium RNA Cycloheximide Edetic Acid Ribonucleases Adenine Fluorides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rosemond H
Sreevalsan T
References (46)
46 references, click to expand
  1. A NEW METHOD FOR THE DETERMINATION OF THE BASE COMPOSITION OF RIBONUCLEIC ACID.
    J Biol Chem. 1963 Sep;238:3065-7 PMID: 14081925
  2. APPLICABILITY OF THE HELA (GEY) STRAIN OF HUMAN MALIGNANT EPITHELIAL CELLS TO THE PROPAGATION OF ARBOVIRUSES.
    Proc Soc Exp Biol Med. 1964 Jun;116:354-8 PMID: 14189137
  3. INHIBITION OF PROTEIN SYNTHESIS BY CYCLOHEXIMIDE IN RABBIT RETICULOCYTES.
    Biochem Biophys Res Commun. 1965 Feb 3;18:389-95 PMID: 14300754
  4. Constitution and function of polyribosomes of poliovirus-infected HeLa cells.
    Virology. 1965 Sep;27(1):44-53 PMID: 4157608
  5. The evaluation of standard sedimentation coefficients of sodium RNA and sodium DNA from sedimentation velocity data in concentrated NaCl and CsCl solutions.
    Biochim Biophys Acta. 1965 Sep 6;108(1):18-29 PMID: 5862239
  6. Polyribosome dissociation and formation in intact reticulocytes with conservation of messenger ribonucleic acid.
    Proc Natl Acad Sci U S A. 1965 Jun;53(6):1437-43 PMID: 5217646
  7. Use of formaldehyde fixation for studies of ribonucleoprotein particles by caesium chloride density-gradient centrifugation.
    J Mol Biol. 1965 Dec;14(2):611-5 PMID: 5326415
  8. Heterogeneous RNA's occurring during the replication of Western equine encephalomyelitis virus.
    Proc Natl Acad Sci U S A. 1966 Apr;55(4):974-81 PMID: 4286764
  9. Buoyant densities of cytoplasmic ribonucleoprotein particles of mammalian cells: distinctive character of ribosome subunits and the rapidly labeled components.
    J Mol Biol. 1966 Apr;16(2):255-68 PMID: 5956031
  10. Device for sectioning of polyacrylamide gel cylinders and its use in determining biological activity in the sections.
    Anal Biochem. 1966 Jun;15(3):544-8 PMID: 5334069
  11. Replication of viral ribonucleic acid. IX. Properties of double-stranded RNA from Escherichia coli infected with bacteriophage MS2.
    J Mol Biol. 1966 May;17(1):145-73 PMID: 5335753
  12. The effect of HeLa cell cytoplasm on the rate of sedimentation of RNA.
    Proc Natl Acad Sci U S A. 1966 Sep;56(3):999-1002 PMID: 4291229
  13. Biosynthesis of immunoglobulins: the separate classes of polyribosomes synthesizing heavy and light chains.
    J Mol Biol. 1967 Jan 28;23(2):201-16 PMID: 6031716
  14. Evidence for a naF-resistant association between mRNA and ribosomes in rabbit reticulocytes.
    Biochim Biophys Acta. 1967 Mar 29;138(1):186-8 PMID: 6057660
  15. In vitro translation of multistranded RNA from Escherichia coli infected by bacteriophage f-2.
    Proc Natl Acad Sci U S A. 1968 Mar;59(3):972-9 PMID: 4868221
  16. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
    Biochemistry. 1968 Feb;7(2):668-74 PMID: 4868544
  17. Ribonucleic acids from animal cells.
    Bacteriol Rev. 1968 Sep;32(3):262-90 PMID: 4176425
  18. Role of ribosomal subunits in protein synthesis in mammalian cells.
    Proc Natl Acad Sci U S A. 1968 Oct;61(2):651-8 PMID: 5245996
  19. Replicative intermediate of an arbovirus.
    J Virol. 1968 Jun;2(6):547-52 PMID: 5748743
  20. Replication of Western equine encephalomyelitis virus. I. Some chemical and physical characteristics of viral ribonucleic acid.
    J Virol. 1968 Jun;2(6):558-66 PMID: 5697470
  21. Maturation defects in temperature-sensitive mutants of Sindbis virus.
    J Virol. 1968 Jul;2(7):728-37 PMID: 5723529
  22. Replication of Western equine encephalomyelitis virus. II. Cytoplasmic structure involved in the synthesis and development of the virions.
    J Virol. 1968 Oct;2(10):1038-46 PMID: 5723707
  23. Sindbis virus infection of chick and hamster cells: synthesis of virus-specific proteins.
    Virology. 1969 Mar;37(3):367-76 PMID: 5777557
  24. Analysis of poliovirus-specific RNA in infected HeLa cells by polyacrylamide gel electrophoresis.
    Virology. 1969 Apr;37(4):535-44 PMID: 4305632
  25. he effect of inhibitors of protein synthesis on the level of ribosomal subunits in ascites cells.
    Biochim Biophys Acta. 1969 May 20;182(1):264-6 PMID: 5792854
  26. Sindbis virus-induced viral ribonucleic acid polymerase.
    J Virol. 1969 Jun;3(6):599-604 PMID: 5798245
  27. Initiation of polyribosome formation in poliovirus-infected HeLa cells.
    J Mol Biol. 1970 Feb 14;47(3):275-91 PMID: 4313849
  28. Effect of sodium fluoride on protein synthesis in HeLa cells: inhibition of ribosome dissociation.
    J Mol Biol. 1970 Feb 14;47(3):335-52 PMID: 5418164
  29. Virus nucleic acids formed in chick embryo cells infected with Semliki Forest virus.
    J Gen Virol. 1970 Feb;6(2):231-48 PMID: 5461804
  30. Virus-specific proteins synthesized in cells infected with RNA+ temperature-sensitive mutants of Sindbis virus.
    J Virol. 1970 Mar;5(3):329-37 PMID: 5461887
  31. Protein synthesis directed by an arbovirus.
    J Virol. 1968 Jan;2(1):26-32 PMID: 5754569
  32. Interferon action on parental Semliki forest virus ribonucleic acid.
    J Virol. 1967 Dec;1(6):1168-73 PMID: 5621488
  33. Purification and properties of Semliki Forest virus nucleocapsids.
    Virology. 1970 Jun;41(2):306-20 PMID: 5463618
  34. Cytoplasmic fractions associated with Semliki Forest virus ribonucleic acid replication.
    J Virol. 1967 Apr;1(2):374-83 PMID: 5630381
  35. Molecular weight of Sindbis virus ribonucleic acid as measured by polyacrylamide gel electrophoresis.
    J Virol. 1970 Jul;6(1):145-7 PMID: 5471476
  36. Association of viral ribonucleic acid with cellular membranes in chick embryo cells infected with Sindbis virus.
    J Virol. 1970 Oct;6(4):438-44 PMID: 5497889
  37. Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA.
    Science. 1971 Oct 29;174(4008):507-10 PMID: 5110429
  38. Analysis of arbovirus ribonucleic acid forms by polyacrylamide gel electrophoresis.
    J Virol. 1971 Apr;7(4):504-14 PMID: 5165207
  39. Functional defects of temperature-sensitive mutants of Sindbis virus.
    J Mol Biol. 1968 Jul 14;35(1):193-205 PMID: 5761396
  40. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321-5 PMID: 5288381
  41. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331-5 PMID: 5288382
  42. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336-40 PMID: 5288383
  43. An adenylate-rich segment in the virion RNA of Sindbis virus.
    Biochem Biophys Res Commun. 1972 Jan 31;46(2):712-8 PMID: 5061927
  44. Isolation and identification of the virus-specified RNA species found on membrane-bound polyribosomes of chick embryo cells infected with Semliki Forest virus.
    Biochem Biophys Res Commun. 1972 Sep 5;48(5):1254-8 PMID: 4341056
  45. Inhibition of viral RNA synthesis by parafluorophenylalanine.
    Proc Natl Acad Sci U S A. 1961 Mar 15;47:278-82 PMID: 13783680
  46. Separation of labeled from unlabeled proteins by equilibrium density gradient sedimentation.
    Anal Biochem. 1962 Dec;4:489-504 PMID: 13955327
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.11.3.399-415.1973
Published
1973-03-00
Pages
399-415
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355115
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.11.3.399-415.1973 PMC Full Text View journal details
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