The mutability of stem bromelain: evidence for perturbation by structural transitions of the parameters that characterize the reaction of the essential thiol group of bromelain with 2,2'-dipyridyl disulphide.

The Biochemical journal ·Vol. 128 ·No. 4 ·1972-07-00 ·Pages 979-82

Brocklehurst K, Crook EM, Kierstan M

Abstract

暂无摘要

MeSH Terms

Bromelains Chromatography Disulfides Hydrogen-Ion Concentration Protein Conformation Pyridines Sulfhydryl Compounds

Chemicals

Disulfides Pyridines Sulfhydryl Compounds Bromelains

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Brocklehurst K

Crook E M

Kierstan M

References (6)

6 references, click to expand

A novel reactivity of papain and a convenient active site titration in the presence of other thiols.
FEBS Lett. 1970 Jul 29;9(2):113-116 PMID: 11947645
The preparation and some properties of bromelain covalently attached to O-(carboxymethyl)-cellulose.
Eur J Biochem. 1968 Dec 5;6(4):565-71 PMID: 5701971
PURIFICATION AND PHYSICAL CHARACTERIZATION OF STEM BROMELAIN.
Biochemistry. 1964 Jan;3:48-55 PMID: 14114503
KINETIC STUDIES OF BROMELAIN CATALYSIS.
Biochemistry. 1963 Nov-Dec;2:1439-44 PMID: 14093923
Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasioned by its interaction with the histidine-159-asparagine-175 hydrogen-bonded system.
Biochem J. 1972 Jun;128(2):471-4 PMID: 5084800
Evidence for glycoprotein nature of stem bromelain. Isolation of a glycopeptide.
Biochemistry. 1967 Dec;6(12):3730-6 PMID: 6076623

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

Published

1972-07-00

Pages

979-82

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1173919

Subset

External Links

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