Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasioned by its interaction with the histidine-159-asparagine-175 hydrogen-bonded system.

The Biochemical journal ·Vol. 128 ·No. 2 ·1972-06-00 ·Pages 471-4

Brocklehurst K, Little G

Abstract

暂无摘要

MeSH Terms

Asparagine Cysteine Disulfides Histidine Hydrogen-Ion Concentration Papain Pyridines Sulfhydryl Compounds

Chemicals

Disulfides Pyridines Sulfhydryl Compounds Histidine Asparagine Papain Cysteine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Brocklehurst K

Little G

References (5)

5 references, click to expand

A novel reactivity of papain and a convenient active site titration in the presence of other thiols.
FEBS Lett. 1970 Jul 29;9(2):113-116 PMID: 11947645
KINETICS OF PAPAIN-CATALYZED HYDROLYSIS OF ALPHA-N-BENZOYL-L-ARGININE ETHYL ESTER AND ALPHA-N-BENZOYL-L-ARGININAMIDE.
J Am Chem Soc. 1965 Jun 20;87:2728-37 PMID: 14292172
IV. Cysteine proteinases. The structure of the papain molecule.
Philos Trans R Soc Lond B Biol Sci. 1970 Feb 12;257(813):231-6 PMID: 4399047
Evidence for histidine in the active site of papain.
Biochem J. 1968 Aug;108(5):855-9 PMID: 5673530
Kinetics of the reversible reaction of papain with 5,5'-dithiobis-(2-nitrobenzoate) dianion: evidence for nucleophilic reactivity in the un-ionized thiol group of cysteine-25 and for general acid catalysis by histidine-159 of the reaction of the 5-mercapto-2-nitrobenzoate dianion with the papain-5-mercapto-2-nitrobenzoate mixed disulphide.
Biochem J. 1972 Jun;128(2):475-7 PMID: 5084801

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

Published

1972-06-00

Pages

471-4

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1173786

Subset

External Links

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