Kinetics of the reversible reaction of papain with 5,5'-dithiobis-(2-nitrobenzoate) dianion: evidence for nucleophilic reactivity in the un-ionized thiol group of cysteine-25 and for general acid catalysis by histidine-159 of the reaction of the 5-mercapto-2-nitrobenzoate dianion with the papain-5-mercapto-2-nitrobenzoate mixed disulphide.

The Biochemical journal ·Vol. 128 ·No. 2 ·1972-06-00 ·Pages 475-7

Little G, Brocklehurst K

Abstract

暂无摘要

MeSH Terms

Benzoates Catalysis Cysteine Disulfides Histidine Kinetics Nitro Compounds Papain Sulfhydryl Compounds Sulfides

Chemicals

Benzoates Disulfides Nitro Compounds Sulfhydryl Compounds Sulfides Histidine Papain Cysteine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Little G

Brocklehurst K

References (4)

4 references, click to expand

Reaction of protein disulfide groups with Ellman's reagent: a case study of the number of sulfhydryl and disulfide groups in Aspergillus oryzae -amylase, papain, and lysozyme.
Arch Biochem Biophys. 1971 Nov;147(1):262-9 PMID: 5114933
Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasioned by its interaction with the histidine-159-asparagine-175 hydrogen-bonded system.
Biochem J. 1972 Jun;128(2):471-4 PMID: 5084800
A novel reactivity of papain and a convenient active site titration in the presence of other thiols.
FEBS Lett. 1970 Jul 29;9(2):113-116 PMID: 11947645
The effect of pH on the affinities of enzymes for substrates and inhibitors.
Biochem J. 1953 Aug;55(1):161-70 PMID: 13093634

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

Published

1972-06-00

Pages

475-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1173787

Subset

External Links

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