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PMID: 4725038 Published · ppublish English Journal Article

Glyceride lipases in nerve endings of guinea-pig brain and their stimulation by noradrenaline, 5-hydroxytryptamine and adrenaline.

The Biochemical journal ·Vol. 132 ·No. 2 ·1973-02-00 ·Pages 233-48

Vyvoda OS, Rowe CE

Abstract

1. Combined guinea-pig cortex and cerebellum was shown to contain triglyceride lipase, diglyceride lipase and monoglyceride lipase, which were assayed by the release of [1-(14)C]palmitate from [1-(14)C]palmitoylglycerol esters. Triglyceride lipase and diglyceride lipase were found in all particulate fractions. 2. With osmotically ruptured synaptosomes the rates of release of palmitate from glyceryl tripalmitate and glyceryl dipalmitate were 7-25mumol/h per g of protein and 0.18-0.69mmol/h per g of protein respectively. The logarithm of the rate of hydrolysis of glyceryl monopalmitate increased linearly with the logarithm of protein concentration. The pH optima of triglyceride lipase and diglyceride lipase were between 7 and 8. The pH optimum for monoglyceride lipase was approx. 8. 3. Triglyceride lipase and diglyceride lipase of osmotically ruptured synaptosomes were stimulated by noradrenaline, 5-hydroxytryptamine and adrenaline. Triglyceride lipase of isolated synaptic membranes was stimulated by 0.01-1mm-noradrenaline. Aging of membranes at 0 degrees C decreased activity, which could still be stimulated by noradrenaline. Diglyceride lipase of isolated membranes was stimulated by 1mum-1mm-noradrenaline. The activity of triglyceride lipase in isolated synaptic vesicles was diminished by 1mm-5-hydroxytryptamine.

MeSH Terms
Animals Brain/cytology,drug effects,enzymology Carbon Isotopes Epinephrine/pharmacology Female Glycerides Guinea Pigs Hydrogen-Ion Concentration Kinetics Lipase/metabolism Membranes/enzymology Nerve Endings/drug effects,enzymology Norepinephrine/pharmacology Palmitic Acids/metabolism Serotonin/pharmacology Subcellular Fractions/enzymology Synaptosomes/drug effects,enzymology Time Factors Triglycerides/metabolism
Chemicals
Carbon Isotopes Glycerides Palmitic Acids Triglycerides Serotonin Lipase Norepinephrine Epinephrine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vyvoda O S
Rowe C E
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55 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1320233
Published
1973-02-00
Pages
233-48
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1177582
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1320233 PMC Full Text View journal details
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