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PMID: 4758368 Published · ppublish English Journal Article

Radioimmunoassay of tumour specific transplantation antigen of a chemically induced rat sarcoma: circulating soluble tumour antigen in tumour bearers.

British journal of cancer ·Vol. 28 ·No. 5 ·1973-11-00 ·Pages 377-88

Thomson DM, Sellens V, Eccles S, Alexander P

Abstract

The tumour specific transplantation antigen (TSTA) from a chemically induced rat sarcoma has been isolated as an electrophoretically homogeneous soluble material by affinity chromatography using a Sepharose bound antibody raised to the tumour in syngeneic rats. The TSTA is specific for the particular tumour used (the MC-1 sarcoma) and does not cross-react with material extracted from other rat sarcomata. In addition, a material with different physicochemical properties which cross-reacted with different sarcomata was also eluted from the antibody column and this may be the previously identified onco-embryonic antigen (OEA1) which is immunogenic in the syngeneic host.The purified TSTA labelled with (125)I was used in a radioimmunoassay which detected soluble TSTA in rats bearing a MC-1 sarcoma. The assay shows that tumour transplantation is associated with a persisting release of soluble antigen into the circulation. This antigenic burden is present continuously and renewed as long as the tumour mass exists.

MeSH Terms
Animals Antibodies, Neoplasm Antigens, Neoplasm/analysis Chromatography, Affinity Cross Reactions Electrophoresis, Polyacrylamide Gel Immune Sera Iodine Isotopes Neoplasm Transplantation Radioimmunoassay Rats Sarcoma, Experimental/immunology
Chemicals
Antibodies, Neoplasm Antigens, Neoplasm Immune Sera Iodine Isotopes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Thomson D M
Sellens V
Eccles S
Alexander P
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28 references, click to expand
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/bjc.1973.163
Published
1973-11-00
Pages
377-88
Language
English
Region
England
NLM ID
0370635
PMCID
PMC2008920
Subset
IM
External Links
PubMed source DOI: 10.1038/bjc.1973.163 PMC Full Text View journal details
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