Clonal nature of the immune response to phosphorylcholine (PC). V. Cross-idiotypic specificity among heavy chains of murine anti-PC antibodies and PC-binding myeloma proteins.

The Journal of experimental medicine ·Vol. 141 ·No. 5 ·1975-05-01 ·Pages 1073-83

Claflin JL, Davie JM

Abstract

Seven mouse myeloma proteins with specificity for phosphorylcholine (PC) were found to share a common antigenic determinant. This group of proteins contained members which differed in genetic origin, heavy chain class, kappa-chain subgroup, individual antigenic determinants and specificity for choline analogues. The cross-idiotypic determinant, VH-PC, was antigenically similar in each of the proteins and was associated with the variable portion of the heavy chain in the region of the antibody combining site. Further studies showed that an indistinguishable determinant was present on IgM anti-PC antibodies isolated from all strains of mice tested regardless of histocompatibility or heavy chain allotype. In view of the finding that this cross-idiotypic determinant was not found on antibodies or myeloma proteins which lacked specificity for PC, the data strongly suggest that a particular heavy chain variable region has been preserved in all mouse antibodies with specificity for PC.

MeSH Terms

Animals Antibody Formation Antibody Specificity Binding Sites, Antibody Choline/analogs & derivatives,immunology Clone Cells Cross Reactions Epitopes Haptens Immunogenetics Immunoglobulin Fragments Immunoglobulin M Immunoglobulins Iodine Radioisotopes Mice Mice, Inbred AKR Mice, Inbred BALB C Mice, Inbred Strains Myeloma Proteins Phosphorus Protein Binding Rabbits/immunology

Chemicals

Epitopes Haptens Immunoglobulin Fragments Immunoglobulin M Immunoglobulins Iodine Radioisotopes Myeloma Proteins Phosphorus Choline

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Claflin J L

Davie J M

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31 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.141.5.1073

Published

1975-05-01

Pages

1073-83

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2189783

Subset

External Links

PubMed source DOI: 10.1084/jem.141.5.1073 PMC Full Text View journal details

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Clonal nature of the immune response to phosphorylcholine (PC). V. Cross-idiotypic specificity among heavy chains of murine anti-PC antibodies and PC-binding myeloma proteins.

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