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PMID: 480476 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

RNA-directed DNA synthesis in Moloney murine leukemia virus: interaction between the primer tRNA and the genome RNA.

Journal of virology ·Vol. 31 ·No. 2 ·1979-08-00 ·Pages 398-407

Peters G, Dahlberg JE

Abstract

Initiation of RNA-directed DNA synthesis in virions of Moloney murine leukemia virus requires a cellular tRNAPro as primer. The site(s) on the Moloney murine leukemia virus genome RNA at which functional primer molecules are bound and at which purified tRNAPro hybridizes has been located near (within 20%) the 5' end of the genome. A relatively stable duplex (temperature at which 50% dissociation has occurred, 76 degrees C) is formed between the amino acid acceptor stem of the tRNAPro and a complementary sequence in the Moloney murine leukemia virus 35S RNA. The interaction involves 19 base pairs, extending from the penultimate nucleotide at the 3' end of the tRNAPro but apparently not including the 3'-terminal adenosine residue. In most respects, the interaction between primer and template in Moloney murine leukemia virus parallels the situation in the avian leukosis-sarcoma viruses.

MeSH Terms
Animals Binding Sites Cell Line DNA, Viral/biosynthesis Fibroblasts Genes, Viral Hot Temperature Mice Moloney murine leukemia virus/genetics,metabolism Nucleic Acid Denaturation Oligonucleotides/analysis RNA, Transfer/metabolism RNA, Viral/analysis,metabolism
Chemicals
DNA, Viral Oligonucleotides RNA, Viral RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Peters G
Dahlberg J E
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33 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.31.2.398-407.1979
Published
1979-08-00
Pages
398-407
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353462
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.31.2.398-407.1979 PMC Full Text View journal details
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