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PMID: 4833608 Published · ppublish English Journal Article

Polyadenylate sequences on Newcastle disease virus mRNA synthesized in vivo and in vitro.

Journal of virology ·Vol. 13 ·No. 6 ·1974-06-00 ·Pages 1220-30

Weiss SR, Bratt MA

Abstract

Polyadenylate [poly(A)] sequences are associated with the 35 and 50S Newcastle disease virus (NDV)-specific RNAs as well as all six to seven of the 18-22S NDV-specific messenger RNAs extracted from infected chicken embryo cells. The poly(A) associated with the 18-22S RNA has an average size of 120 to 130 nucleotides. The 18-22S RNA synthesized in vitro by NDV's virion-bound polymerase contains six to seven species of the same size and relative proportions as its intracellular counterpart. This in vitro synthesized 18-22S RNA also contains covalently linked poly(A) sequences which, although variable in size, are usually larger and more heterogeneous than those from the infected cell. In vitro RNA synthesis is supported not only by magnesium (at an optimal concentration of mM) but by manganese (at an optimal concentration of 0.5 to 1.0 mM) as well. However, the major product made in the presence of manganese, although sedimenting at 18 to 22S, differs somewhat from the product made in the presence of magnesium.

MeSH Terms
Adenosine Alkalies Animals Base Sequence Cells, Cultured Chick Embryo Electrophoresis, Polyacrylamide Gel Newcastle disease virus/analysis Oligonucleotides/analysis RNA, Messenger/analysis RNA, Viral/analysis Ribonucleases Tritium Uridine
Chemicals
Alkalies Oligonucleotides RNA, Messenger RNA, Viral Tritium Ribonucleases Adenosine Uridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weiss S R
Bratt M A
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30 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.13.6.1220-1230.1974
Published
1974-06-00
Pages
1220-30
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355441
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.13.6.1220-1230.1974 PMC Full Text View journal details
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