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PMID: 4357880 Published · ppublish English Journal Article

In vitro synthesis of RNA that contains polyadenylate by virion-associated RNA polymerase of vesicular stomatitis virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 12 ·1973-12-00 ·Pages 3566-70

Banerjee AK, Rhodes DP

Abstract

The RNA synthesized in vitro by the virion-associated RNA-instructed RNA polymerase of purified vesicular stomatitis virus contains polyadenylate sequences. These have been demonstrated by their partial resistance to pancreatic and T(1) ribonucleases and their capacity to bind to poly(U) filters and oligo(dT)-cellulose. The polyadenylate sequences range in apparent size from 50 to 200 bases, similar to the size of the poly(A) in mRNA from vesicular stomatitis virus-infected cells. Possible mechanisms of polyadenylylation of the in vitro RNA product are discussed.

MeSH Terms
Adenine Nucleotides/analysis,metabolism Adenosine/metabolism Base Sequence Binding Sites Centrifugation, Density Gradient DNA-Directed RNA Polymerases/metabolism Electrophoresis, Polyacrylamide Gel Hydrolysis In Vitro Techniques Nucleic Acid Hybridization Phosphorus Radioisotopes Polynucleotides/analysis RNA, Viral/analysis,biosynthesis,isolation & purification Ribonucleases Thymine Nucleotides Transcription, Genetic Tritium Uracil Nucleotides/metabolism Vesicular stomatitis Indiana virus/enzymology
Chemicals
Adenine Nucleotides Phosphorus Radioisotopes Polynucleotides RNA, Viral Thymine Nucleotides Uracil Nucleotides Tritium DNA-Directed RNA Polymerases Ribonucleases Adenosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Banerjee A K
Rhodes D P
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.12.3566
Published
1973-12-00
Pages
3566-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427281
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.12.3566 PMC Full Text View journal details
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