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PMID: 4833611 Published · ppublish English Journal Article

Origin of reovirus oligo(A).

Journal of virology ·Vol. 13 ·No. 6 ·1974-06-00 ·Pages 1331-7

Carter C, Stoltzfus CM, Banerjee AK, Shatkin AJ

Abstract

Reovirus contains about 1,200 molecules per virion of oligo(A) of chain length 10 to 15 nucleotides in addition to the 10 double-stranded genome segments. Virions purified from infected BHK, HeLa, or L cells had similar amounts of oligo(A) of the same composition, indicating that it is a virus-specific product. Although conversion of virions to cores by chymotryptic digestion resulted in an almost complete loss of oligo(A) and a marked decrease in infectivity, the infectivity could be partially restored by adsorbing cores to cells in the presence of Kaopectate. Core-infected cells yielded virions that contained a normal complement of oligo(A). The results indicate that oligo(A) is not essential for infectivity or required as a primer/template for its own synthesis.

MeSH Terms
Adenosine Animals Cell Line Chromatography, DEAE-Cellulose Chromatography, Gel Chymotrypsin Cricetinae HeLa Cells Kaolin Kidney L Cells Oligonucleotides/analysis Phosphorus Radioisotopes RNA, Viral/analysis Reoviridae/analysis Tritium
Chemicals
Oligonucleotides Phosphorus Radioisotopes RNA, Viral Tritium Kaolin Chymotrypsin Adenosine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Carter C
Stoltzfus C M
Banerjee A K
Shatkin A J
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32 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.13.6.1331-1337.1974
Published
1974-06-00
Pages
1331-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355453
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.13.6.1331-1337.1974 PMC Full Text View journal details
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