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PMID: 493145 Published · ppublish English Journal Article

Extrachromosomal circular ribosomal DNA in the yeast Saccharomyces carlsbergensis.

Nucleic acids research ·Vol. 7 ·No. 1 ·1979-09-11 ·Pages 69-76

Meyerink JH, Klootwijk J, Planta RJ, van der Ende A, van Bruggen EF

Abstract

Purified ribosomal DNA from Saccharomyces carlsbergensis contains a small proportion of circular DNA molecules with a contour length of 3 micron or integral multiples thereof. Hybridization of yeast ribosomal DNA with 26 S rRNA, using the R-loop technique, reveals that these circular molecules contain sequences complementary to yeast ribosomal RNA. We suggest that these extrachromosomal rRNA genes may be intermediates in the amplification of rRNA genes in yeast.

MeSH Terms
DNA, Circular/analysis Microscopy, Electron Molecular Weight Nucleic Acid Conformation Nucleic Acid Hybridization Ribosomes/analysis Saccharomyces/analysis
Chemicals
DNA, Circular
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Meyerink J H
Klootwijk J
Planta R J
van der Ende A
van Bruggen E F
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.1.69
Published
1979-09-11
Pages
69-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327996
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.1.69 PMC Full Text View journal details
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