Isolation and analysis of the protoplast membrane of Bacillus megaterium.

The Biochemical journal ·Vol. 98 ·No. 3 ·1966-03-00 ·Pages 923-8

Yudkin MD

Abstract

1. Optimum conditions were found for the lysis of Bacillus megaterium KM by lysozyme. The age of culture, density of suspension and concentration of lysozyme affected the rate of lysis. 2. Protoplast membranes were isolated by centrifugation of lysates and were exhaustively washed. 3. Treatment with chloroform removed some lipid from the membranes, but about half of the total membrane lipid could be extracted only after partial acid hydrolysis. 4. The defatted membranes consisted of protein together with variable amounts of RNA; carbohydrate was almost absent. 5. Lipid accounted for 23% of the weight of the membrane, and included both neutral lipid and phospholipid. In both classes, branched-chain C(15) acids made up about 80% of the total fatty acid. 6. The phospholipid was a kephalin, and contained small quantities of several amino acids.

MeSH Terms

Amino Acids Bacillus megaterium/cytology Chemistry Techniques, Analytical Chromatography, Gas Fatty Acids Lipids Muramidase Phospholipids Protoplasts

Chemicals

Amino Acids Fatty Acids Lipids Phospholipids Muramidase

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Yudkin M D

References (31)

31 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0980923

Published

1966-03-00

Pages

923-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1264938

Subset

External Links

PubMed source DOI: 10.1042/bj0980923 PMC Full Text View journal details

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Isolation and analysis of the protoplast membrane of Bacillus megaterium.

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