Fluorescent method for the detection of excreted ribonuclease around bacterial colonies.

Journal of bacteriology ·Vol. 92 ·No. 5 ·1966-11-00 ·Pages 1469-72

Lanyi JK, Lederberg J

Abstract

Lanyi, Janos K. (Stanford University School of Medicine, Palo Alto, Calif.), and Joshua Lederberg. Fluorescent method for the detection of excreted ribonuclease around bacterial colonies. J. Bacteriol. 92:1469-1472. 1966.-A test for the release of extracellular ribonuclease by Bacillus subtilis colonles was developed. The method consists of incorporating acridine orange and ribonucleic acid into nutrient agar plates and viewing the grown bacterial colonies under ultraviolet light. Regions of ribonuclease secretion appear as dark halos around the colonies on a green fluorescent background. The theoretical basis and the utility of this test are discussed.

MeSH Terms

Acridines Bacillus subtilis/analysis Culture Media Microscopy, Fluorescence RNA, Bacterial Ribonucleases/analysis

Chemicals

Acridines Culture Media RNA, Bacterial Ribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lanyi J K

Lederberg J

References (12)

12 references, click to expand

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Proc Natl Acad Sci U S A. 1959 Jul;45(7):944-52 PMID: 16590488
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.92.5.1469-1472.1966

Published

1966-11-00

Pages

1469-72

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC276447

Subset

External Links

PubMed source DOI: 10.1128/jb.92.5.1469-1472.1966 PMC Full Text View journal details

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Fluorescent method for the detection of excreted ribonuclease around bacterial colonies.

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