Adenosine triphosphate-dependent synthesis of biologically active DNA by azide-poisoned bacteria.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 6 ·1971-06-00 ·Pages 1296-300

Ganesan AT

Abstract

Nonviable cells of Bacillus subtilis, when made permeable by treatment under controlled conditions with nonionic detergent, can be shown to replicate DNA normally and to repair certain regions of the chromosome. The former process is stimulated by ATP in the presence of dATP, dCTP, dGTP, and dTTP. The product, a result of semiconservative replication, is biologically active; synthesis of the newly-formed regions of the chromosome appears to be sequential.

MeSH Terms

Adenosine Triphosphate/pharmacology Autoradiography Azides/pharmacology Bacillus subtilis/drug effects,metabolism Carbon Isotopes Cell Membrane Permeability/drug effects DNA Replication/drug effects DNA, Bacterial/analysis Detergents/pharmacology Molecular Weight Nucleosides/metabolism Nucleotides/metabolism Thymidine/metabolism Tritium

Chemicals

Azides Carbon Isotopes DNA, Bacterial Detergents Nucleosides Nucleotides Tritium Adenosine Triphosphate Thymidine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Ganesan A T

References (10)

10 references, click to expand

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Proc Natl Acad Sci U S A. 1970 Oct;67(2):674-81 PMID: 4943178
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Proc Natl Acad Sci U S A. 1970 Oct;67(2):773-8 PMID: 4943181
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Proc Natl Acad Sci U S A. 1963 Jan 15;49:12-7 PMID: 13997384
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.6.1296

Published

1971-06-00

Pages

1296-300

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389176

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.6.1296 PMC Full Text View journal details

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Adenosine triphosphate-dependent synthesis of biologically active DNA by azide-poisoned bacteria.

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