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PMID: 5002281 Published · ppublish English Journal Article

In vitro excision-repair of ultraviolet-irradiated transforming DNA from Bacillus subtilis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 12 ·1971-12-00 ·Pages 2967-71

Heijneker HL, Pannekoek H, Oosterbaan RA, Pouwels PH, Bron S, Arwert F, Venema G

Abstract

The biological activity of UV-inactivated Bacillus subtilis DNA is partly restored after incubation with a UV-specific endonuclease from Micrococcus lutens in conjunction with DNA polymerase and DNA ligase, both isolated from Escherichia coli. The restored activity is not further increased by photoreactivation. Pyrimidine dimers are specifically liberated when irradiated DNA is exposed to the three enzymes. None of these effects is observed when pancreatic DNase is used instead of UV-specific endonuclease.

MeSH Terms
Bacillus subtilis/enzymology,metabolism Cells, Cultured DNA Nucleotidyltransferases/metabolism DNA Repair DNA, Bacterial/radiation effects Darkness Deoxyribonucleases/metabolism Enzyme Activation Escherichia coli/enzymology Ligases/metabolism Micrococcus/enzymology Pancreas/enzymology Photochemistry Polymers/metabolism Pyrimidines/metabolism Radiation Effects Transformation, Genetic Ultraviolet Rays
Chemicals
DNA, Bacterial Polymers Pyrimidines DNA Nucleotidyltransferases Deoxyribonucleases Ligases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Heijneker H L
Pannekoek H
Oosterbaan R A
Pouwels P H
Bron S
Arwert F
Venema G
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.12.2967
Published
1971-12-00
Pages
2967-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389571
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.12.2967 PMC Full Text View journal details
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