The deoxyribonucleic acid modification enzyme of bacteriophage P1.
The bacteriophage P1 modification enzyme, assayed by the specific methylation of unmodified bacteriophage 82 DNA, has been purified 500-fold from a bacteriophage P1 lysogen of Escherichia coli. The enzyme catalyses the incorporation of approximately 20-24 methyl groups per bacteriophage 82 DNA molecule. The sole product of methylation is 6-methylaminopurine. Methylation of unmodified bacteriophage DNA confers protection against a challenge by purified bacteriophage P1 restriction enzyme. The pH optimum is 6.0-6.25: the apparent K(m) for S-adenosyl-l-methionine is 5x10(-6)m.
22 references, click to expand
-
Restriction of infectious bacteriophage fd DNA's and an assay for in vitro host-controlled restriction and modification.Proc Natl Acad Sci U S A. 1968 Apr;59(4):1294-9 PMID: 5240030
-
Host specificity of DNA produced by Escherichia coli, X. In vitro restriction of phage fd replicative form.Proc Natl Acad Sci U S A. 1968 Apr;59(4):1300-6 PMID: 4870862
-
Host specificity of DNA produced by Escherichia coli. XI. In vitro modification of phage fd replicative form.Proc Natl Acad Sci U S A. 1969 Jun;63(2):556-62 PMID: 4895540
-
Recognition of nucleotide sequences.Annu Rev Biochem. 1969;38:841-80 PMID: 4896249
-
DNA modification and restriction.Annu Rev Biochem. 1969;38:467-500 PMID: 4897066
-
The Escherichia coli B restriction endonuclease.Biochim Biophys Acta. 1969 Nov 19;195(1):219-29 PMID: 4901831
-
A specific complex between a restriction endonuclease and its DNA substrate.Proc Natl Acad Sci U S A. 1970 Feb;65(2):357-62 PMID: 4984237
-
Clear plaque mutants of phage P1.Virology. 1970 May;41(1):66-71 PMID: 5444276
-
Complementation analysis of temperature-sensitive host specificity mutations in Escherichia coli.J Mol Biol. 1970 May 28;50(1):111-27 PMID: 4916553
-
A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.J Mol Biol. 1970 Jul 28;51(2):379-91 PMID: 5312500
-
DNA restriction enzyme from E. coli.Nature. 1968 Mar 23;217(5134):1110-4 PMID: 4868368
-
A restriction enzyme from Hemophilus influenzae. II.J Mol Biol. 1970 Jul 28;51(2):393-409 PMID: 5312501
-
DNA methylation of T-even bacteriophages and of their nonglucosylated mutants: its role in P1-directed restriction.Virology. 1970 Oct;42(2):359-67 PMID: 5489224
-
Structure of the DNA of bacteriophage phiX174. VII. Methylation.J Mol Biol. 1970 Oct 28;53(2):251-9 PMID: 5493279
-
Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants.J Mol Biol. 1968 Jul 14;34(2):331-46 PMID: 4938550
-
On the assay, isolation and characterization of the lac repressor.J Mol Biol. 1968 Jul 14;34(2):361-4 PMID: 4938551
-
DNA-glucosylation in T-even phage: genetic determination and role in phagehost interaction.Annu Rev Genet. 1970;4(0):177-92 PMID: 4950809
-
Protein measurement with the Folin phenol reagent.J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
-
Suppression of the multiplication of heterologous bacteriophages in lysogenic bacteria.Virology. 1957 Jun;3(3):496-513 PMID: 13443211
-
The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg.J Mol Biol. 1960 Dec;2:392-415 PMID: 13750787
-
Transduction of linked genetic characters of the host by bacteriophage P1.Virology. 1955 Jul;1(2):190-206 PMID: 13267987
-
Host specificity of DNA produced by Escherichia coli. I. Host controlled modification of bacteriophage lambda.J Mol Biol. 1962 Jul;5:18-36 PMID: 13862047
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