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PMID: 5128334 Published · ppublish English Journal Article

Oxidation of nicotinic acid by a Bacillus species: purification and properties of nicotinic acid and 6-hydroxynicotinic acid hydroxylases.

Journal of bacteriology ·Vol. 108 ·No. 2 ·1971-11-00 ·Pages 751-6

Hirschberg R, Ensign JC

Abstract

The enzymes of a Bacillus species that hydroxylate nicotinic acid to 6-hydroxynicotinic acid and 6-hydroxynicotinic acid to 2,6-dihydroxynicotinic acid were purified and characterized. The purified enzymes contained approximately two molecules of flavine and eight molecules of iron per molecule of enzyme. The enzymes were large (molecular weight, 400,000 to 450,000) and appeared to consist of subunits.

MeSH Terms
Ammonium Sulfate Bacillus/enzymology,growth & development,metabolism Cell-Free System Chemical Precipitation Chromatography, DEAE-Cellulose Chromatography, Gel Culture Media Electrophoresis Flavins/analysis Gels Hydroxylation Iron/analysis Mixed Function Oxygenases/analysis,isolation & purification,metabolism Molecular Weight Nicotinic Acids/metabolism Spectrophotometry Ultracentrifugation
Chemicals
Culture Media Flavins Gels Nicotinic Acids Iron Mixed Function Oxygenases Ammonium Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hirschberg R
Ensign J C
References (18)
18 references, click to expand
  1. The hydroxylation of nicotine: the origin of the hydroxyl oxygen.
    Biochem Biophys Res Commun. 1965 Dec 21;21(6):644-8 PMID: 5879471
  2. Nicotinic acid metabolism. II. The isolation and characterization of intermediates in the fermentation of nicotinic acid.
    J Biol Chem. 1966 Apr 25;241(8):1807-13 PMID: 5945854
  3. The purification and properties of nicotine oxidase.
    Biochim Biophys Acta. 1967 May 16;139(1):56-68 PMID: 4962139
  4. Nicotinic acid metabolism. 3. Purification and properties of a nicotinic acid hydroxylase.
    J Biol Chem. 1969 Mar 10;244(5):1194-203 PMID: 4388026
  5. Nicotinic acid metabolism. IV. Ferredoxin-dependent reduction of 6-hydroxynicotinic acid to 6-oxo-1,4,5,6-tetrahydronicotinic acid.
    J Biol Chem. 1969 Mar 10;244(5):1204-11 PMID: 5767303
  6. Purification and characterization of a multienzyme complex in the tryptophan pathway of Neurospora crassa.
    J Biol Chem. 1969 May 25;244(10):2716-25 PMID: 5769998
  7. Oxidation of nicotinic acid by a Bacillus species: source of oxygen atoms for the hydroxylation of nicotinic acid and 6-hydroxynicotinic acid.
    J Bacteriol. 1971 Nov;108(2):757-9 PMID: 5128335
  8. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  9. 6-Hydroxynicotinic acid as an intermediate in the oxidation of nicotinic acid by Pseudomonas fluorescens.
    Biochem J. 1955 Jun;60(2):303-10 PMID: 14389240
  10. Bacterial fermentation of nicotinic acid. I. End products.
    J Biol Chem. 1957 Aug;227(2):815-22 PMID: 13463003
  11. Bacterial fermantation of nicotinic acid. II. Anaerobic reversible hydroxylation of nicotinic acid to 6-hydroxynicotinic acid.
    J Biol Chem. 1957 Aug;227(2):823-31 PMID: 13463004
  12. The bacterial oxidation of nicotinic acid.
    J Biol Chem. 1957 Oct;228(2):923-45 PMID: 13475371
  13. Purification of the nicotinic acid hydroxylase system of Pseudomonas fluorescens KB1.
    Biochem J. 1959 May;72(1):1-7 PMID: 13651127
  14. Simplified methods for the estimation of iron in mitochondria and submitochondrial fractions.
    Arch Biochem Biophys. 1962 Apr;97:37-40 PMID: 13886880
  15. alpha-Keto acid dehydrogenation complexes. IV. Resolution and reconstitution of the Escherichia coli pyruvate dehydrogenation complex.
    J Biol Chem. 1963 Jan;238:30-9 PMID: 14034257
  16. NICOTINIC ACID METABOLISM. I. DISTRIBUTION OF ISOTOPE IN FERMENTATION PRODUCTS OF LABELLED NICOTINIC ACID.
    J Biol Chem. 1964 Mar;239:902-6 PMID: 14157025
  17. THE PATHWAY OF NICOTINIC ACID OXIDATION BY A BACILLUS SPECIES.
    J Biol Chem. 1964 Jul;239:2285-91 PMID: 14216418
  18. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
    Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.108.2.751-756.1971
Published
1971-11-00
Pages
751-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247135
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.108.2.751-756.1971 PMC Full Text View journal details
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