Oxidation of nicotinic acid by a Bacillus species: source of oxygen atoms for the hydroxylation of nicotinic acid and 6-hydroxynicotinic acid.

Journal of bacteriology ·Vol. 108 ·No. 2 ·1971-11-00 ·Pages 757-9

Hirschberg R, Ensign JC

Abstract

Three types of evidence are presented to show that the enzymes that hydroxylate nicotinic acid to 2,6-dihydroxynicotinic acid use water as a source of oxygen atoms. (18)O is incorporated into the products from H(2) (18)O. Molecular oxygen acts as a terminal electron acceptor, one-half molecule being consumed per molecule of hydroxyl groups incorporated. An external electron acceptor is required for activity in purified preparations.

MeSH Terms

Bacillus/enzymology,growth & development,metabolism Cell-Free System Chemistry, Organic Chromatography, DEAE-Cellulose Culture Media Electron Transport Hydroxylation Mass Spectrometry Mixed Function Oxygenases/isolation & purification,metabolism Nicotinic Acids/metabolism Organic Chemistry Phenomena Oxygen Oxygen Consumption Oxygen Isotopes Water/metabolism

Chemicals

Culture Media Nicotinic Acids Oxygen Isotopes Water Mixed Function Oxygenases Oxygen

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hirschberg R

Ensign J C

References (5)

5 references, click to expand

The hydroxylation of nicotine: the origin of the hydroxyl oxygen.
Biochem Biophys Res Commun. 1965 Dec 21;21(6):644-8 PMID: 5879471
Nicotinic acid metabolism. IV. Ferredoxin-dependent reduction of 6-hydroxynicotinic acid to 6-oxo-1,4,5,6-tetrahydronicotinic acid.
J Biol Chem. 1969 Mar 10;244(5):1204-11 PMID: 5767303
Nature and mechanisms of oxygenases.
Science. 1969 Apr 25;164(3878):389-96 PMID: 4304952
THE PATHWAY OF NICOTINIC ACID OXIDATION BY A BACILLUS SPECIES.
J Biol Chem. 1964 Jul;239:2285-91 PMID: 14216418
Oxidation of nicotinic acid by a Bacillus species: purification and properties of nicotinic acid and 6-hydroxynicotinic acid hydroxylases.
J Bacteriol. 1971 Nov;108(2):751-6 PMID: 5128334

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.108.2.757-759.1971

Published

1971-11-00

Pages

757-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247136

Subset

External Links

PubMed source DOI: 10.1128/jb.108.2.757-759.1971 PMC Full Text View journal details

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Oxidation of nicotinic acid by a Bacillus species: source of oxygen atoms for the hydroxylation of nicotinic acid and 6-hydroxynicotinic acid.

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