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PMID: 5166238 Published · ppublish English Journal Article

Microbial transformation of antibiotics: phosphorylation of clindamycin by Streptomyces coelicolor Müller.

Journal of bacteriology ·Vol. 108 ·No. 1 ·1971-10-00 ·Pages 459-64

Coats JH, Argoudelis AD

Abstract

Addition of clindamycin to whole-cell cultures of Streptomyces coelicolor Müller resulted in the loss of in vitro activity against organisms sensitive to clindamycin. Incubation of such culture filtrates with alkaline phosphatase generated a biologically active material identified as clindamycin. Fermentation broths containing inactivated clindamycin yielded clindamycin 3-phosphate, the structure of which was established by physical-chemical and enzymatic studies. Clindamycin was phosphorylated by lysates and partially purified enzyme preparations from S. coelicolor Müller. These reactions require a ribonucleoside triphosphate and Mg(2+). The product of the cell-free reactions was identified as clindamycin 3-phosphate.

MeSH Terms
Adenosine Triphosphate/metabolism Alkaline Phosphatase/pharmacology Ammonium Sulfate Animals Anti-Bacterial Agents/isolation & purification,metabolism,pharmacology Biological Assay Cattle Cell-Free System Chemical Phenomena Chemistry Chlorides Chromatography, DEAE-Cellulose Chromatography, Thin Layer Culture Media Enzymes/isolation & purification,metabolism Fermentation Glycosides/metabolism Hydrolysis Infrared Rays Intestines/enzymology Lincomycin/metabolism Magnesium Magnetic Resonance Spectroscopy Manganese Nucleotides/metabolism Oxidative Phosphorylation Pyrrolidines/metabolism Sarcina/drug effects Spectrophotometry Streptomyces/enzymology,metabolism Temperature
Chemicals
Anti-Bacterial Agents Chlorides Culture Media Enzymes Glycosides Nucleotides Pyrrolidines Manganese Adenosine Triphosphate Lincomycin Alkaline Phosphatase Magnesium Ammonium Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Coats J H
Argoudelis A D
References (15)
15 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.108.1.459-464.1971
Published
1971-10-00
Pages
459-64
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247086
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.108.1.459-464.1971 PMC Full Text View journal details
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