Changes in the physical state of DNA during replication in regenerating liver of the rat.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 65 ·No. 1 ·1970-01-00 ·Pages 152-9

Berger H, Irvin JL

Abstract

Evidence is presented which demonstrates that DNA synthesis in regenerating liver is discontinuous. In pulse-labeling experiments from 30 seconds to 10 minutes, the earliest detectable intermediate in DNA replication appears to be double-stranded DNA which has a significant degree of single-stranded character probably due to the existence of gaps in the newly synthesized strand. The label begins to move into completely double-stranded DNA 10-30 minutes after the commencement of labeling. The average molecular weight of the single-stranded "unit" of DNA replication is determined to be approximately 7.5 million. Smaller units may be produced but not observed as a result of imperfect synchronization of DNA synthesis.

MeSH Terms

Animals Carbon Isotopes Cellulose Centrifugation, Density Gradient Centrifugation, Zonal Chromatography, Ion Exchange DNA/biosynthesis,isolation & purification DNA Replication Hepatectomy Hydroxyapatites Liver/metabolism Liver Regeneration Male Molecular Weight Nitrates Rats Tritium

Chemicals

Carbon Isotopes Hydroxyapatites Nitrates Tritium Cellulose DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Berger H

Irvin J L

References (11)

11 references, click to expand

Chromatography of nucleic acids on hydroxyapatite.
Nature. 1965 May 22;206(4986):779-83 PMID: 5840127
Tables for estimating sedimentation through linear concentration gradients of sucrose solution.
Anal Biochem. 1967 Jul;20(1):114-49 PMID: 6034985
Isolation of deoxyribonucleic acid from mammalian tissues.
Biochem J. 1967 Jul;104(1):254-7 PMID: 4291998
Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.
Proc Natl Acad Sci U S A. 1968 Feb;59(2):598-605 PMID: 4967086
Studies of DNA replication in vivo. I. Isolation of the first intermediate of DNA replication in bacteria as single-stranded DNA.
Proc Natl Acad Sci U S A. 1968 May;60(1):329-36 PMID: 4968634
Polydeoxynucleotide intermediates in DNA replication in regenerating liver.
Nature. 1968 Oct 12;220(5163):162-4 PMID: 5684823
DNA replication in vivo by a temperature-sensitive polynucleotide ligase mutant of T4.
Proc Natl Acad Sci U S A. 1968 Nov;61(3):997-1004 PMID: 5246564
Studies on the nature of replicating DNA of HeLa cells.
Biochim Biophys Acta. 1969 Jan 21;174(1):253-63 PMID: 5766295
Early events in the replication and integration of DNA into mammalian chromosomes.
Biochem Biophys Res Commun. 1969 Feb 7;34(3):291-300 PMID: 5767023
Artefact in the measurement of the molecular weight of pulse labelled DNA.
Nature. 1969 Mar 15;221(5185):1053-6 PMID: 5774397
SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.
J Mol Biol. 1965 Feb;11:373-90 PMID: 14290352

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.65.1.152

Published

1970-01-00

Pages

152-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC286204

Subset

External Links

PubMed source DOI: 10.1073/pnas.65.1.152 PMC Full Text View journal details

Analysis Services

Changes in the physical state of DNA during replication in regenerating liver of the rat.

Contact

WeChat Official Account