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PMID: 5288252 Published · ppublish English Journal Article

Translation of exogenous messenger RNA for hemoglobin on reticulocyte and liver ribosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 11 ·1971-11-00 ·Pages 2752-6

Prichard PM, Picciano DJ, Laycock DG, Anderson WF

Abstract

The ribosome and initiation factor requirements for translation of rabbit-reticulocyte hemoglobin mRNA on rabbit reticulocyte ribosomes, reticulocyte ribosomal subunits, and liver ribosomes have been studied. Excellent synthesis of globin chains from exogenous mRNA in the fractionated cell-free system has been achieved. There is a near absolute requirement for each of the initiation factors, M(1), M(2), and M(3) (as well as for the supernatant proteins) for the translation of exogenous mRNA. Liver microsomal wash will partially replace reticulocyte factors M(1) and M(2), but will not replace the requirement for reticulocyte factor M(3). Rabbit liver ribosomes and rabbit reticulocyte ribosomes are equally active in their ability to support the translation of exogenous hemoglobin mRNA.

MeSH Terms
Animals Cell-Free System Chromatography, DEAE-Cellulose Globins/biosynthesis Hemoglobins/biosynthesis Liver/cytology,metabolism Microsomes, Liver/metabolism Peptide Chain Initiation, Translational RNA, Messenger/biosynthesis,metabolism Rabbits Reticulocytes/metabolism Ribosomes/metabolism
Chemicals
Hemoglobins RNA, Messenger Globins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Prichard P M
Picciano D J
Laycock D G
Anderson W F
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.11.2752
Published
1971-11-00
Pages
2752-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389517
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.11.2752 PMC Full Text View journal details
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