Initial dipeptide formation in hemoglobin biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 8 ·1971-08-00 ·Pages 1810-4

Crystal RG, Shafritz DA, Prichard PM, Anderson WF

Abstract

Initiation factors M(1) + M(2) from reticulocyte ribosomes bind Met-tRNA(F) to rabbit reticulocyte ribosomes containing endogenous hemoglobin mRNA. The initial binding of Met-tRNA(F) appears to be to the small ribosomal subunit. The Met-tRNA(F) is able to participate in what is presumed to be the first peptide bond in the formation of hemoglobin, namely the synthesis of a methionyl-valine dipeptide. The formation of this methionyl-valine dipeptide requires Met-tRNA(F), initiation factors M(1), M(2), and M(3), as well as Val-tRNA and T(1). No synthesis of methionyl-valine dipeptide takes place if Met-tRNA(F) is replaced by Met-tRNA(M), or if initiation factor M(3) is omitted. Thus, Met-tRNA(F) appears to be the initiator tRNA for hemoglobin biosynthesis and M(3), although required for the synthesis of the first peptide bond of hemoglobin, does not appear to be necessary, under the experimental conditions studied, for Met-tRNA(F) binding.

MeSH Terms

Animals Binding Sites Centrifugation, Density Gradient Chromatography, DEAE-Cellulose Dipeptides/biosynthesis Electrophoresis Formates Hemoglobins/biosynthesis Magnesium/pharmacology Methionine/metabolism Peptide Chain Initiation, Translational RNA, Transfer/analysis,metabolism Rabbits Receptors, Drug Reticulocytes/analysis,cytology,metabolism Ribosomes/metabolism Sulfur Isotopes Valine/metabolism

Chemicals

Dipeptides Formates Hemoglobins Receptors, Drug Sulfur Isotopes RNA, Transfer Methionine Valine Magnesium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Crystal R G

Shafritz D A

Prichard P M

Anderson W F

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28 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.8.1810

Published

1971-08-00

Pages

1810-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389298

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.8.1810 PMC Full Text View journal details

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Initial dipeptide formation in hemoglobin biosynthesis.

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