A bovine pancreatic enzyme catalyzing the conversion of proinsulin to insulin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 6 ·1971-06-00 ·Pages 1312-5

Yip CC

Abstract

An enzyme that catalyzes the conversion of bovine proinsulin to insulin has been purified from a bovine pancreatic extract. The product of conversion was identified as insulin by aminoacid analysis and determination of carboxyl terminal aminoacid residues. The purified enzyme preparation showed one major protein band on polyacrylamide gel disc electrophoresis; it had a molecular weight of about 70,000 and an isoelectric point (pI) at a pH of 4.82.

MeSH Terms

Amino Acids/analysis Animals Biotransformation Carboxypeptidases/pharmacology Cattle Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Electrophoresis, Disc Enzymes/isolation & purification,pharmacology Hydrolysis Insulin/analysis,metabolism Molecular Weight Pancreas/enzymology Pancreatic Extracts/analysis Proinsulin/analysis,metabolism Zinc/pharmacology

Chemicals

Amino Acids Enzymes Insulin Pancreatic Extracts Proinsulin Carboxypeptidases Zinc

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Yip C C

References (14)

14 references, click to expand

Biosynthesis of insulin in anglerfish islets.
Nature. 1970 Feb 7;225(5232):548-9 PMID: 4904263
Kinetics of the tryptic hydrolysis of zinc-free bovine insulin.
J Biol Chem. 1969 Oct 25;244(20):5537-43 PMID: 5388596
Carboxy-peptidase B. 4. Purification and characterization of the porcine enzyme.
J Biol Chem. 1960 Aug;235:2272-7 PMID: 13823740
Biosynthesis of insulin in bovine fetal pancreatic slices: the incorporation of tritiated leucine into a single-chain proinsulin, a double-chain intermediate, and insulin in subcellular fractions.
Proc Natl Acad Sci U S A. 1969 Jun;63(2):442-9 PMID: 5257135
Porcine proinsulin: characterization and amino acid sequence.
Science. 1968 Jul 12;161(3837):165-7 PMID: 5657063
Insulin biosynthesis: evidence for a precursor.
Science. 1967 Aug 11;157(3789):697-700 PMID: 4291105
Interaction of zinc with proinsulin.
Biochem Biophys Res Commun. 1970 Jan 23;38(2):284-9 PMID: 5418703
The biosynthesis of insulin and a probable precursor of insulin by a human islet cell adenoma.
Proc Natl Acad Sci U S A. 1967 Feb;57(2):473-80 PMID: 16591494
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Isolation and properties of proinsulin, intermediate forms, and other minor components from crystalline bovine insulin.
Diabetes. 1968 Dec;17(12):725-36 PMID: 5726253
Conversion of proinsulin to insulin in a subcellular fraction from rat islets.
Biochem Biophys Res Commun. 1970 Dec 9;41(5):1223-30 PMID: 4921757
Subcellular localization of proinsulin to insulin conversion in isolated rat islets.
Endocrinology. 1970 Jan;86(1):88-96 PMID: 4901349
A specific anti-proinsulin serum and the presence of proinsulin in calf serum.
Proc Natl Acad Sci U S A. 1969 Feb;62(2):415-9 PMID: 5256220
The biosynthesis of insulin and "proinsulin" in foetal bovine pancreas.
Diabetologia. 1968 Jan;4(1):68-70 PMID: 5741527

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.6.1312

Published

1971-06-00

Pages

1312-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389179

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.6.1312 PMC Full Text View journal details

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A bovine pancreatic enzyme catalyzing the conversion of proinsulin to insulin.

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